Anti-Neuroligin-1 Antibody (N97A/31)

Name: Anti-Neuroligin-1 Antibody
Brand: NeuroMab
SKU: 75-160
Price: 337.0 USD
Availability: InStock

Our mouse Neuroligin-1 monoclonal antibody from NeuroMab is produced in-house from clone N97A/31. It detects chicken, mouse, and rat Neuroligin-1 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Adult rat hippocampus immunohistochemistry.

Immunoblot against crude membranes from adult mouse brain (MBM), extracts of COS cells transiently transfected with Neuroligin-1, -2, -3, -4 or -4* plasmid and extracts of brains from wild-type (WT) and Neuroligin-1 knockout (KO) mice probed with N97A/31 TC supe. Data courtesy of Marc Bolliger and Tom Sudhof (Stanford).

Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N97A/31 (left) or N52A/42 (right) TC supe.

Confocal microscopy analysis of NLGN1(red) and APC (magenta) co-staining in HuTu 80 cells. Image from publication CC-BY-4.0. PMID:36056393

Adult rat brain membrane immunoblot.

Click on image to zoom

Species Reactivity

Chicken, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-160

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Neuroligin-1

Scientific Background

Neuroligin 1 is a member of a large family of alternatively sliced proteins located at the post synaptic surface in the central nervous system, and stains as a post synaptic marker. Neuroligin 1 is involved in cell-cell-interactions by binding to neurexin family members and other postsynaptic proteins to localize receptors and channels in the postsynaptic density. Suggested roles for Neuroligin 1 include synapse function and synaptic signal transmission and maintaining synapse integrity. Mutations in Neuroligans have been implicated in autism and other cognitive disorders.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N97A/31

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Nlgn1

Molecular Weight

120 kDa

Immunogen

Fusion protein amino acids 718-843 (intracellular C-terminus) of rat Neuroligin-1 (accession number NP_446320) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Chicken, Mouse, Rat

Antibody Registry ID

AB_2235964

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.86

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with Neuroligin-2, -3, -4 or -4*

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Neuroligin-1 (Neuroligin I)

UniProt ID

Q62765

Shipping

Shipped on ice packs

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Anti-Neuroligin-1 Antibody (N97A/31)

SKU: 75-160

Product Details

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