Anti-Mortalin/GRP75 Antibody (N52A/42)

Name: Anti-Mortalin/GRP75 Antibody
Brand: NeuroMab
SKU: 75-127
Price: 337.0 USD
Availability: InStock

Our mouse Mortalin/GRP75 monoclonal antibody from NeuroMab is produced in-house from clone N52A/42. It detects C. elegans, human, mouse, non-human primate, and rat Mortalin/GRP75 and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Immunoblot against crude membranes prepared from whole rat (RBM) and mouse (MBM) brain and human cerebral cortex [HBM(Cx)], cerebellum [HBM(Cb)], and hippocampus [HBM(H)] probed with N52A/42 TC supe.

Adult rat hippocampus immunohistochemistry.

Immunoblot against crude C. elegans worm extracts and RBM probed with N52A/42 TC supe.

Immunoblot against HEK293 cells transfected as shown. Note that GRP75 is endogenously expressed in HEK (and COS) cells and the relatively weak RBM staining is due to short film exposure time. Cell samples courtesy of Gino Cortopassi (UC Davis).

$Western blot showing immunoblotting of cytochrome c oxidase I (COXI), cytochrome c oxidase IV (COXIV), GRP75/mortalin, VDAC1, MFN2 , and calnexin in various mouse whole brain fraction. ER (endoplasmic reticulum); CM (crude mitochondria); PM (pure mitochondria); MAM (mitochondria-associated membranes). Image from publication CC-BY-4.0. PMID: 35943566$

Immunoblot against crude membranes prepared from whole rat (RBM) and mouse (MBM) brain and human cerebral cortex [HBM(Cx)], cerebellum [HBM(Cb)], and hippocampus [HBM(H)] probed with N52A/42 TC supe.

Click on image to zoom

Species Reactivity

C. elegans, Human, Mouse, Non-Human Primate, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-127

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Mortalin/GRP75

Scientific Background

Mitochondrial stress-70 protein or Mortalin/GRP75 is encoded by the gene HSPA9. Mortalin is a chaperone protein and plays a role in cell proliferation, stress response and mitochondria maintenance. It is widely expressed in tissues in mitochondria, endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. Diseases associated with this gene include Even-Plus Syndrome and Sideroblastic Anemia 4.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N52A/42

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Hspa9 Grp75 Hsp74 Hspa9a

Molecular Weight

75 kDa

Immunogen

Identified as off-target mAb in screen for anti-Salm2 NeuroMabs (immunogen was fusion protein amino acids 551-766 of mouse SALM2). Target identified as Mortalin/Grp75 by LC-MS/MS analysis of protein immunopurified from rat brain using N52A/42.

Immunogen Species

Mouse

Species Reactivity

C. elegans, Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2120479

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.54

Dilution Ranges

WB: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Stress-70 protein, mitochondrial (75 kDa glucose-regulated protein) (GRP-75) (Heat shock 70 kDa protein 9) (Mortalin) (Peptide-binding protein 74) (PBP74) (p66 MOT)

UniProt ID

P38647

Shipping

Shipped on ice packs

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Anti-Mortalin/GRP75 Antibody (N52A/42)

SKU: 75-127

Product Details

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