Our mouse Kvbeta1.1 K+ channel FL650 conjugate monoclonal antibody from NeuroMab is produced in-house from clone K9/40. It detects human, mouse, non-human primate, and rat Kvbeta1.1 K+ channel and is purified by Protein A chromatography. KO validated. Works in IHC, ICC.
Voltage-gated potassium channel subunit beta-1 or Kvβ1.1 is encoded by the gene KCNAB1. Kvß1.1 is a member of the potassium channel, voltage-gated, shaker-related subfamily. Alternative splicing of KCNAB1 allows for three distinct proteins to be made, Kvß1.1, Kvß1.2, and Kvß1.3. Each of these has a unique N terminus. Kvβ1.1 is a cytoplasmic subunit that modulates the characteristics of the membrane spanning, channel-forming alpha-subunits. Beta subunits can promote the closure of channels or enhance channel activity depending on the channel members. Kvß1 is predominantly expressed in brain and found in hippocampus, cerebral cortex, caudate putamen, colliculus and cerebellum. Diseases associated with this gene include Episodic Ataxia, Type 1 and Familial Temporal Lobe Epilepsy, 3
Purified by Protein A chromatography
Lot dependent: provided at 0.3-0.5 mg/mL
Monoclonal
K9/40
IgG2b
ICC, IHC
Mouse
Kcnab1 Kvb1
44 kDa
Synthetic peptide amino acids 7-28 (unique N-terminus) of rat Kv1.1 (TEHNLKSRNGEDRLLSKQSST; accession number NP_058999)
Rat
Human, Mouse, Non-Human Primate, Rat
AB_2939135
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
FL650 Ex: 655 nm, Em: 676 nm
Does not cross-react with Kvβ1.2, Kvβ1.3, Kvβ2
Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
KO Validated