Anti-Kv1.2 K+ Channel Antibody (K14/16)

Our mouse Kv1.2 K+ channel monoclonal antibody from NeuroMab is produced in-house from clone K14/16. It detects gerbil, human, mouse, non-human primate, rat, Xenopus, and zebrafish Kv1.2 K+ channel and is purified by Protein A chromatography. KO validated. Works in WB, IHC, ICC, IP, ELISA.

Immunolabeling of postnatal 14 mouse CA3 (Axonal Initial Segment, Parvalbumin positive interneuron) labeling Kv1.2 (75-008, 1:200, yellow) Parvalbumin (magenta), and Ankyrin G (cyan). Mice were transcardially perfused with cold 4% PFA in PBS, then brain removed and postfixed overnight. 50 uM slices as floating sections, antigen retrieval performed with sodium citrate at 80 C for 30 min. before immunolabeling. Images from J. Ramirez-Franco and O. El Far, Aix-Marseille Université and INSERM.
Immunolabeling of permeabilized HEK293 cells specifically labeling intracellular Kv1.2 (Cat. 75-008, 1:200, red) and DAPI labelling DNA (blue). Images kindly provided by Ivan Talucci and Maric Hans, Rudolf Virchow Center for Integrative and Translational Bioimaging.
Immunoblot on brain membranes from wild-type (WT) and Kv1.2 knockout (Kv1.2-/-) mice and from adult rat brain (RBM).
Immunolabeling of permeabilized mouse teased fibers, where in red is the juxta-paranodal staining for Kv1.2 (Cat. 75-008, 1:200, red). Murine sciatic nerves were dissected from hind limbs, fixed in 4% paraformaldehyde, and washed in PBS. The epineurium was removed prior to teasing. Teased fibers were air-dried and stored at −20°C until further usage. Obj. 63x, scale bar = 10um. Images kindly provided by Ivan Talucci and Maric Hans, Rudolf Virchow Center for Integrative and Translational Bioimaging.
Adult rat hippocampus immunohistochemistry.
Adult rat brain membrane immunoblot.
Immunoblot on membranes from whole rat (RBM) and mouse (MBM) brain and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].
Immunofluorescence showing labeling of CASPR (red) and Kv1.2(green) in the spinal cord of P21 Shank3(+ / +) and Shank3Δ11(−/−) mice. Image from publication CC-BY-4.0. PMID: 35726031
Binding of serum samples 1, 3, 27, healthy control (HC) serum and a commercial antibody against the according Kv channels to transfected HEK293 cells. Cells were transfected with human Kv1.2 and stained with a Kv1.2 antibody (Cat. 75-008, 1:200; red). Binding of patient serum was visualized with anti-human-IgG-Cy3 antibody (green). Image from publication CC-BY-4.0. PMID:38665908
Left, 63× images of Kv1.2 immunoreactivity at CA3 Axonal Initial segments in WT (top panels) and Lgi1−/− (bottom panels). Scale bar = 10 μm. Right, detail of a CA3 pyramidal neuron AIS in WT (top panels) and Lgi1−/− (bottom panels).
63× images showing no differences in Kv1.2 IR at the AIS of PV+ interneurons. Scale bar = 10 μm (G) Cumulative probability plots of Kv1.2 immunoreactivity at individual AIS of WT (black squares) and Lgi1−/− (red circles) CA3 pyramidal neurons
Immunolabeling of postnatal 14 mouse CA3 (Axonal Initial Segment, Parvalbumin positive interneuron) labeling Kv1.2 (75-008, 1:200, yellow) Parvalbumin (magenta), and Ankyrin G (cyan). Mice were transcardially perfused with cold 4% PFA in PBS, then brain removed and postfixed overnight. 50 uM slices as floating sections, antigen retrieval performed with sodium citrate at 80 C for 30 min. before immunolabeling. Images from J. Ramirez-Franco and O. El Far, Aix-Marseille Université and INSERM.
Immunolabeling of postnatal 14 mouse CA3 (Axonal Initial Segment, Parvalbumin positive interneuron) labeling Kv1.2 (75-008, 1:200, yellow) Parvalbumin (magenta), and Ankyrin G (cyan). Mice were transcardially perfused with cold 4% PFA in PBS, then brain removed and postfixed overnight. 50 uM slices as floating sections, antigen retrieval performed with sodium citrate at 80 C for 30 min. before immunolabeling. Images from J. Ramirez-Franco and O. El Far, Aix-Marseille Université and INSERM.
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Gerbil, Human, Mouse, Non-Human Primate, Rat, Xenopus, Zebrafish
ELISA, ICC, IHC, IP, WB
Mouse
490
550
594
650
Biotin
Carrier-Free

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Data Sheet
KO Validated KO Validated Trial Size Trial Size

SKU: 75-008

Volume: 100 µL
Price:
$394.00
Ships: 1-2 business days

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