Anti-GluN2B/NR2B Glutamate Receptor Antibody (N59/36)

Name: Anti-GluN2B/NR2B Glutamate Receptor Antibody
Brand: NeuroMab
SKU: 75-101
Price: 394.0 USD
Availability: InStock

Our mouse GluN2B/NR2B glutamate receptor monoclonal antibody from NeuroMab is produced in-house from clone N59/36. It detects human, mouse, and rat GluN2B/NR2B glutamate receptor and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Cultured rat hippocampal neuron immunofluorescence. Image courtesy of Anthone Dunah (Harvard) and Morgan Sheng (MIT).

Immunoblot against rat brain membranes (RBM) and neuronal lysates from NR2B knockout (KO) and heterozygote (Het) mice. Samples courtesy of Ben Hall and Anirvan Ghosh (UCSD).

Adult rat hippocampus immunohistochemistry.

Immunostaining of rat dentate gyrus section labeled with Anti-GluN2B/NR2B Glutamate Receptor (Cat. 75-101, 48 hr, 1:1000, 4 C) Following transcardial perfusion of rat with 4% (w/v) paraformaldehyde, brain was post fixed for 48 hours, cut to 30 microns, and free-floating sections were labeled. For visualization, sections were labeled with biotinylated horse anti-mouse followed with 0.05% (w/v) glucose oxidase DAB and 4.167% NiSO4. Image provided by Josh Allen, University of Victoria.

Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].

Immunostaining for GFAP, NR2B (Cat. 75-101, 1:100), and TH expression indicates proper differentiation of six-week-old neuronal cultures. Image from publication CC-BY-4.0. PMID:37739965

Representative images showing immunostaining of PSD95, synaptophysin (Syph), Vglut1, Vglut2, Vgat, and NR2b (Cat. 75-101, 1:100) in the lateral nuclei of the amygdala (LA) of WT and Slitrk4 KO mice. Image from publication CC-BY-4.0. PMID:38736483

Anti-GluN2B/NR2B Glutamate Receptor Antibody

Cultured rat hippocampal neuron immunofluorescence. Image courtesy of Anthone Dunah (Harvard) and Morgan Sheng (MIT).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

Trial Size

SKU: 75-101

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GluN2B/NR2B glutamate receptor

Scientific Background

GluN2B/NR2b glutamate receptor, also known as Glutamate/N-methyl D-aspartate/NMDA receptor subtype 2B, is a member of the NMDA receptor family. NMDA receptors are expressed throughout the CNS in postsynaptic cell membranes. GluN2B is a major NMDAR subtype found in cortical and hippocampal regions of the adult brain. NMDA receptors are involved with memory and learning. Mutations in the gene encoding GluN2B are associated with autism, LennoxGastaut and West Syndromes.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N59/36

Isotype

IgG2b

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Grin2b

Molecular Weight

166 kDa

Immunogen

Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B (accession number Q00960) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2232584

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.28

Dilution Ranges

WB: 1:1000
IHC: 1:1000
ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with GluN2A/NR2A

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Glutamate receptor ionotropic, NMDA 2B (GluN2B) (Glutamate [NMDA] receptor subunit epsilon-2) (N-methyl D-aspartate receptor subtype 2B) (NMDAR2B) (NR2B)

UniProt ID

Q00960

Shipping

Shipped on ice packs

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Anti-GluN2B/NR2B Glutamate Receptor Antibody (N59/36)

SKU: 75-101

Product Details

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