Products: Primary Antibodies

Anti-VSP/TPTE Antibody (N432/63)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-486	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-486	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Chicken Xenopus Zebrafish

Applications:

Target Description:

TPTEs (transmembrane phosphatases with tensin homology) aka VSPs (voltage sensing phosphatases) catalyze the dephosphorylation of phosphatidylinositol phosphates (PIPs) that regulate diverse aspects of cell membrane physiology including cell division, growth and migration (Ratzan W. et al., 2019). TPTEs are also thought to be involved in signal transduction pathways of the endocrine or spermatogenetic function of the testis (Chen H. et al., 1999).

Immunogen:

Fusion protein amino acids 153-335 of zebrafish TPTE (also known as Transmembrane phosphatase with tensin homology, voltage-sensing phosphoinositide phosphatase and VSP, accession number Q4V9E4)

Protein Name(s):

Transmembrane phosphatase with tensin homology (voltage-sensing phosphoinositide phosphatase) ( VSP)

Gene Name(s):

TPTE

Antibody Registry ID:

Purified: AB_2744561
TC Supernatant: AB_2716254

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

40-60 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.