Products: Primary Antibodies

Anti-VMAT1 Antibody (N440/61)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-431	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-431	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Mouse Rat

Applications:

ICC IHC

Target Description:

Vesicular monamine transporters (VMATs) sequester neurotransmitters such as dopamine, serotonin and adrenaline into synaptic vesicles (Erickson et al., 1996). Two VMATs have been identified, VMAT1 and VMAT2. VMAT1 shows a higher affinity for serotonin (Brunk et al., 2006). Antibodies specific for VMATs have been used to monitor expression of the transporter during development and in aging and can be effectively used as a marker for monoamine terminals (Haycock et al., 2003; Witkovsky et al., 2005).

Immunogen:

Fusion protein amino acids 43-135 (first lumenal domain) of mouse Slc18a1 (also known as Solute carrier family 18 member 1, Vesicular amine transporter 1, Chromaffin granule amine transporter, VAT1, VMAT1 and Cgat, accession number Q8R090)
Rat: 92% identity (86/93 amino acids identical)
Human: 65% identity (62/96 amino acids identical)
30% identity with Slc18a2/VMAT2

Cross Reactivity:

Does not cross-react with VMAT2

Protein Name(s):

Chromaffin granule amine transporter (Solute carrier family 18 member 1) (Vesicular amine transporter 1) (VAT1)

Gene Name(s):

Slc18a1 Vmat1

Antibody Registry ID:

Purified: AB_2632412
TC Supernatant: AB_2532072

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

100 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.