Products: Primary Antibodies

Anti-VMAT1 (N440/61)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-431	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-431	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Mouse Rat

Applications:

ICC IHC

Immunogen:

Fusion protein amino acids 43-135 (first lumenal domain) of mouse Slc18a1 (also known as Solute carrier family 18 member 1, Vesicular amine transporter 1, Chromaffin granule amine transporter, VAT1, VMAT1 and Cgat, accession number Q8R090)
Rat: 92% identity (86/93 amino acids identical)
Human: 65% identity (62/96 amino acids identical)
30% identity with Slc18a2/VMAT2

Cross Reactivity:

Does not cross-react with VMAT2

Protein Name(s):

Chromaffin granule amine transporter (Solute carrier family 18 member 1) (Vesicular amine transporter 1) (VAT1)

Gene Name(s):

Slc18a1 Vmat1

Antibody Registry ID:

Purified: AB_2632412
TC Supernatant: AB_2532072

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

100 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.