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Products: Primary Antibodies

Anti-VAPA Antibody (N479/22)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-495
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-495
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC WB

Target Description:

VAPA and VAPB are members of the VAMP-associated protein family (VAP) (aka vesicle-associated membrane proteins) which are abundant scaffolding proteins predominantly localized to the endoplasmic reticulum (ER). VAPs have recently been shown to interact with Kv2.1 and Kv2.2 channels forming junctions to link the ER to the plasma membrane (Johnson B. et al., 2018).

Immunogen:

Fusion protein amino acids 1-219 (N-terminus) of rat VAPA (also known as Vesicle-associated membrane protein-associated protein A, VAMP-associated protein A, 33 kDa VAMPassociated protein, VAMP-A, VAP-33 and Vap33, accession number Q9Z270)

Protein Name(s):

VAPA (Vesicle-associated membrane protein-associated protein A) (VAMP-associated protein A) (33 kDa VAMPassociated protein) (VAMP-A) (VAP-33)

Gene Name(s):

Vapa Vap33


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

30 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.