Products: Primary Antibodies

Anti-VAPA/B Antibody (N479/107)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-496	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-496	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Mouse Rat

Applications:

IHC WB

Target Description:

VAPA and VAPB are members of the VAMP-associated protein family (VAP) (aka vesicle-associated membrane proteins) which are abundant scaffolding proteins predominantly localized to the endoplasmic reticulum (ER). VAPs have recently been shown to interact with Kv2.1 and Kv2.2 channels forming junctions to link the ER to the plasma membrane (Johnson B. et al., 2018).

Immunogen:

Fusion protein amino acids 1-219 (N-terminus) of rat VAPA (also known as Vesicle-associated membrane protein-associated protein A, VAMP-associated protein A, 33 kDa VAMPassociated protein, VAMP-A, VAP-33 and Vap33, accession number Q9Z270)

Protein Name(s):

VAPA (Vesicle-associated membrane protein-associated protein A) (VAMP-associated protein A) (33 kDa VAMPassociated protein) (VAMP-A) (VAP-33)

Gene Name(s):

Vapa Vap33

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

30 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.