Products: Primary Antibodies

Anti-Uncx Antibody (N155/9)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-205	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-205	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse

Applications:

ICC WB

Target Description:

Homeobox protein unc-4 homolog is encoded by the gene UNCX. UNCX functions as a transcription factor in somitogenesis and neurogenesis. UNCX is required for the maintenance and differentiation of specific elements of the axial skeleton. UNCX is expressed in the eye, brain and kidney. Diseases associated with UNCX include Autosomal Recessive Spondylocostal Dysostosis.

Immunogen:

Fusion protein amino acids 191-530 (C-terminus) of mouse Uncx (also known as UNC4 protein, Uncx4.1, Transcriptional control factor, Paired-type homeodomain transcription factor and PHD1, accession number O75164); Rat: 97% identity (330/340 amino acids identical); Human: 82% identity (287/350 amino acids identical)

Protein Name(s):

Uncx

Gene Name(s):

Uncx Uncx4.1

Antibody Registry ID:

Purified: AB_10999755
TC Supernatant: AB_10672528

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

55 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.