Products: Primary Antibodies

Anti-TrpC7 (N64A/36)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-123	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-123	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Immunogen:

Synthetic peptide amino acids 845-862 (FGKNLNKDHLRVNKGKDI) of human TrpC7 (also known as short transient receptor potential channel 7, capacitative calcium channel Trp7, accession number Q9HCX4)
Rat: 100% identity (18/18 amino acids identical)
Mouse: 94% identity (17/18 amino acids identical)

Cross Reactivity:

None Reported

Protein Name(s):

Short transient receptor potential channel 7 (TrpC7) (Transient receptor protein 7) (TRP-7) (hTRP7)

Gene Name(s):

TRPC7 TRP7

Antibody Registry ID:

Purified: AB_2208358
TC Supernatant: AB_10672526

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

100 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Inoue M, Harada K, Matsuoka H, Nakamura J, Warashina A.. (2012), 'Mechanisms and roles of muscarinic activation in guinea-pig adrenal medullary cells. .' American Journal of Physiology-Cell Physiology. 10.1152/ajpcell.00147.2012.
Brandao KE, Dell'Acqua ML, Levinson SR.. (), 'A-kinase anchoring protein 150 expression in a specific subset of TRPV1- and CaV 1.2-positive nociceptive rat dorsal root ganglion neurons. .' Journal of Comparative Neurology. 10.1002/cne.22692.