TMB 1-Component HRP Membrane Substrate (SUBM)
A ready-to-use formulation containing TMB in a mildly acidic buffer. This TMB substrate is useful for membrane-based immunoblotting applications using HRP-conjugates for detection. Yields an insoluble, permanent dark blue reaction product.
TMB 1-Component HRP Membrane Substrate (SUBM)
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Ships: 1-2 business days
- Allow product to equilibrate to room temperature (25°C) prior to use.
- Completely cover the membrane surface with the substrate. The substrate will react with peroxidase creating an insoluble permanent dark blue reaction product.
- To stop the reaction, rinse the developed membrane with high quality water.
- For best results, the reaction should be monitored and read before the background staining becomes too intense to distinguish from the positive staining.