Human Mouse Rat
ICC IHC WB
Thyroid hormones are essential for development of the central nervous system and deficits in these hormones during development affects such cognitive functions as learning and memory (Ambrogini et al., 2005; Chan and Kilby, 2000). Thyroid hormones exert their physiological role mainly through binding to specific nuclear receptors including the predominant isoforms of thyroid hormone receptors TRa1, TRa2, TRß1 and TRß2. TRa1, TRß1 and TRß2 bind T3 with high affinity and also bind to thyroid hormone response elements (TREs) on chromatin to regulate the transcriptional processes in several target tissues, including adult rat brain (Constantinou et al., 2005).
Fusion protein amino acids 1-461 (full-length) of human Thyroid hormone receptor beta1 (also known as Nuclear receptor subfamily 1 group A member 2, c-erbA-2, c-erbA-beta, THRB, ERBA2, NR1A2 and THR1, accession number P10828)
Mouse: 96% identity (445/461 amino acids identical)
Rat: 95% identity (442/461 amino acids identical)
75% identity with Thyroid hormone receptor beta
280% identity with Thyroid hormone receptor alpha1
Does not cross-react with Thyroid hormone receptor beta2 or alpha1
Thyroid hormone receptor beta (Nuclear receptor subfamily 1 group A member 2) (c-erbA-2) (c-erbA-beta)
THRB ERBA2 NR1A2 THR1
TC Supernatant: AB_2336917
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.