Products: Primary Antibodies

Anti-Thorase/Atad1 Antibody (N125/10)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-157	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-157	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

ATPase Family AAA Domain-containing 1 is encoded by the gene ATAD1. ATAD1 plays a critical role in regulating the surface expression of AMPA receptors thereby regulating synaptic plasticity, learning, and memory. Diseases associated with ATAD1 include Hyperekplexia 4 and Hyperekplexia.

Immunogen:

Fusion protein amino acids 1-361 (full-length) of mouse Thorase (also known as ATPase family AAA domain-containing protein 1 and Atad1, accession number Q9D5T0); Human: 100% identity (361/361 amino acids identical); Rat: 99% identity (360/361 amino acids identical)

Cross Reactivity:

No cross-reactivity reported

Protein Name(s):

Thorase/Atad1

Gene Name(s):

Atad1

Antibody Registry ID:

Purified: AB_2290002
TC Supernatant: AB_10673514

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

40 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.