Products: Primary Antibodies

Anti-Tauro-GM2 (N175/9)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-191	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Catalog # 73-191	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgM

Applications:

ELISA

Immunogen:

Taurine-conjugated GM2 ganglioside (tauro-GM2) as prepared chemically in 2003 Li et al J BiolChem (PMID 12829702, GM2 in dimethylformamide stirred with taurine, diethyl phosphorocyanidateand triethylamine at room temperature for 5 h, reaction stopped with KCl, mixture passed throughsolid-phase extraction cartridge, unabsorbed materials washed by water and absorbed materialseluted with methanol and further purified by high performance anion-exchange chromatography).

Cross Reactivity:

No cross-reactivity against unconjugated GM2

Protein Name(s):

N/A

Gene Name(s):

N/A

Antibody Registry ID:

Purified: AB_11001847
TC Supernatant: AB_10672373

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Molecular Weight:

None Reported