Products: Primary Antibodies

Anti-Synaptotagmin-9 Antibody (N276A/15)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-306	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-306	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

Synaptotagmins are a family of calcium sensing, membrane trafficking proteins. 17 different synaptotagmins have been identified in mammals. Along with synaptotagmins 1 and 2, synaptotagmin 9 (also known as synaptotagmin 5) has been shown to function as a Ca(2+) sensor for fast synchronous neurotransmitter release (Xu J et al., 2007).

Immunogen:

Fusion protein amino acids 253-344 (cytoplasmic C2B domain) of mouse Synaptotagmin-9 (also known as Synaptotagmin-5, Synaptotagmin V, SytV, Syt5, Synaptotagmin-IX, SytIX and Syt9, accession number Q9R0N5)
Rat: 100% identity (92/92 amino acids identical)
Human: 100% identity (92/92 amino acids identical)>50% identity with Synaptotagmin-1 (and other Synaptotagmins)

Cross Reactivity:

Does not cross-react with Synaptotagmin-1 (or other Synaptotagmins)

Protein Name(s):

Synaptotagmin-5 (Synaptotagmin IX) (Synaptotagmin V) (SytV)

Gene Name(s):

Syt5 Syt9

Antibody Registry ID:

Purified: AB_2315938
TC Supernatant: AB_2315937

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

60 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.