Products: Primary Antibodies

Anti-Synapsin-3 Antibody (L125/121)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-494	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-494	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC WB

Target Description:

The synapsin family of proteins are neuron specific phospho-proteins comprised of synapsins I, II and III. Synapsins I and II are abundantly expressed pre-synaptic proteins that play key roles in synaptic plasticity and synaptogenesis (Feng et al., 2002; Nayak et al., 1996). Synapsin III is the most recent member of the synapsin family to be indentified and is primarily expressed in extra-synaptic sites such as cell bodies and growth cones (Ferreira A. et al., 2000). Synapsin III is expressed at much lower levels than synapsin I and II in the adult brain and is therefore thought to be involved in early neural development (Kao HT, et al., 1998).

Immunogen:

Fusion protein amino acids 76-417 (actin-binding and synaptic-vesicle binding domains) of human Synapsin-III (also known as Synapsin-3 and SYN3, accession number O14994)

Protein Name(s):

Synapsin-III (Synapsin-3)

Gene Name(s):

SYN3

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

60 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.