Products: Primary Antibodies

Anti-Snapin/SNAPAP Antibody (L8/15)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-045	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-045	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Target Description:

SNARE-associated protein Snapin, SNAP Associated Protein or Biogenesis Of Lysosome-Related Organelles Complex 1 Subunit 7 (BLOC-1 Subunit 7) is encoded by the gene SNAPIN. Snapin is a member of the BLOC-1 complex, which is involved in the protein-sorting, membrane biogenesis, and vesicular trafficking. Snapin is required for vesicle docking and fusion and regulates neurotransmitter release. Snapin is expressed in many tissues including heart, brain, testis, kidney and liver. Diseases associated with this gene include Hermansky-Pudlak Syndrome and Tricuspid Valve Stenosis.

Immunogen:

Synthetic peptide of amino acids 115-136 of human snapin (KETARRRAMLDSGIYPPGSPGK; accession number Q95295); Rat: 95% identity (21/22 amino acids); Mouse: 95% identity (21/22 amino acids)

Cross Reactivity:

No cross-reactivity reported

Protein Name(s):

Snapin/SNAPAP

Gene Name(s):

SNAPIN BLOC1S7 SNAP25BP SNAPAP

Antibody Registry ID:

Purified: AB_2255078
TC Supernatant: AB_10675326

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

20 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.