Anti-Slo2.2/Slack K+ Channel Antibody (N3/26)

Name: Anti-Slo2.2/Slack K+ Channel Antibody
Brand: NeuroMab
SKU: 75-051
Price: 374.0 USD
Availability: InStock

35 Citations

Our Anti-Slo2.2/Slack K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N3/26. It is KO validated, detects human, marine mollusk, mouse, and rat Slo2.2/Slack K+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus CA1 immunohistochemistry.

Adult rat medial vestibular nucleus immunohistochemistry.

Adult rat trapezoid body immunohistochemistry.

Immunoblot against brain membranes from adult rat (RBM), mouse (MBM) or human cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] probed with N3/26 (left) or N52A/42 (right) TC supe.

Immunoblot against brain membranes from adult rat (RBM), Slo2.2 knockout (MBM-Slo2.2 KO) or wild-type (MBM-WT) mice probed with N3/26 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Chris Lingle (Washington University).

Double-labeling immunostaining of Slack (red) and TRPV1 (green) in DRGs. Image from publication CC-BY-4.0. PMID: 35626730

Double immunofluorescence staining revealed the expression of the Slack channel (A,D, in red) in somatostatin positive neurons (B,E, in green). Image from publication CC-BY-4.0. PMID:35359569

Adult rat hippocampus CA1 immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-051

Volume: 100 µL

Variant

Price:

$374

Quantity:

Product Details

Target

Slo2.2/Slack K+ channel

Target Description

Potassium channel subfamily T member 1 or -KCNT1/Slo2.2/Slack K+ channel is encoded by the gene KCNT1. Slo2.2 is a member of the the voltage-gated ion channel (vic) superfamily, and the KCNT protein family, which is made of KCNT1 and KCNT2. Slo2.2 functions as an outwardly rectifying potassium channel subunit to contribute to ion conductance and signaling pathways. Slo2.2 can be activated by high intracellular sodium or chloride levels. Slo2.2 is expressed at the membrane in many areas of the brain, brainstem, spinal cord, spleen and liver. Mutations in this gene cause the early-onset epileptic disorders

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N3/26

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Kcnt1 Slack

Molecular Weight

140 kDa

Antigen

Fusion protein amino acids 1168-1237 of rat Slo2.2 (accession number NP_068625) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2131855

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
IHC: 1:400
ICC: 1:250

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Potassium channel subfamily T member 1 (Sequence like a calcium-activated potassium channel subunit)

UniProt Details

UniProt (Human): Q5JUK3
UniProt (Immunogen Species): Q9Z258

Product Specific References for Applications and Species

Immunocytochemistry: Marine Mollusk | Rat
Immunohistochemistry: Mouse | Rat
Immunoprecipitation: Mouse
Western Blot: Mouse | Rat

Immunocytochemistry: Marine Mollusk
PMID	Dilution	Publication
23115170	1:1000	Zhang, Y., et al. 2012. Regulation of neuronal excitability by interaction of fragile X mental retardation protein with slack potassium channels.. Journal of Neuroscience, 15318-15327.

Immunocytochemistry: Rat
PMID	Dilution	Publication
28982974	1:250	Gururaj, S., et al. 2017. Protein kinase A-induced internalization of Slack channels from the neuronal membrane occurs by adaptor protein-2/clathrin-mediated endocytosis. Journal of Biological Chemistry, 19304-19314.
27091544	1:50	Bansal, V., et al. 2016. Na(+) -Activated K(+) Channels in Rat Supraoptic Neurones. Journal of Neuroendocrinology, .
23466807	1:200	Cervantes, B., et al. 2013. Identity, expression and functional role of the sodium-activated potassium current in vestibular ganglion afferent neurons.. Neuroscience, 163-175.
19403831	2.6ug/ml	Chen, H., et al. 2009. The N-terminal domain of Slack determines the formation and trafficking of Slick/Slack heteromeric sodium-activated potassium channels.. Journal of Neuroscience, 5654-5665.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
35626730	1:500	Zhou, F., et al. 2022. Slack Potassium Channels Modulate TRPA1-Mediated Nociception in Sensory Neurons. Cells, .
35359569	1:400	Liu, Y., et al. 2022. The Slack Channel Deletion Causes Mechanical Pain Hypersensitivity in Mice. Frontiers in Molecular Neuroscience, 811441.
35346832	1:400	Gertler, T.S., et al. 2022. KNa1.1 gain-of-function preferentially dampens excitability of murine parvalbumin-positive interneurons. Neurobiology of disease, 105713.
33817875	1:100-1:200	Ehinger, R., et al. 2021. Slack K+ channels attenuate NMDA‐induced excitotoxic brain damage and neuronal cell death.. The FASEB Journal, e21568.
33401689	1:400	Lu, R., et al. 2021. Functional Coupling of Slack Channels and P2X3 Receptors Contributes to Neuropathic Pain Processing. International Journal of Molecular Sciences, 405.
26587966	1:400	Rizzi, S., et al. 2016. Differential distribution of the sodium-activated potassium channels slick and slack in mouse brain.. Journal of Comparative Neurology, 2093-2116.
29124216	1:200	Rizzi, S., et al. 2015. Identification of potential novel interaction partners of the sodium-activated potassium channels Slick and Slack in mouse brain. Biochemistry and Biophysics Reports, 291-298.
25609627	1:400	Lu, R., et al. 2015. Slack channels expressed in sensory neurons control neuropathic pain in mice.. Journal of Neuroscience, 1125-1135.

Immunohistochemistry: Rat
PMID	Dilution	Publication
23872594	1:1000	Huang, F., et al. 2013. TMEM16C facilitates Na(+)-activated K+ currents in rat sensory neurons and regulates pain processing.. Nature Neuroscience, 1284-1290.
23466807	1:200	Cervantes, B., et al. 2013. Identity, expression and functional role of the sodium-activated potassium current in vestibular ganglion afferent neurons.. Neuroscience, 163-175.

Immunoprecipitation: Mouse
PMID	Dilution	Publication
29124216	40ug	Rizzi, S., et al. 2015. Identification of potential novel interaction partners of the sodium-activated potassium channels Slick and Slack in mouse brain. Biochemistry and Biophysics Reports, 291-298.

Western Blot: Mouse
PMID	Dilution	Publication
36173683	8ug/ml	Burbano, L.E., et al. 2022. Antisense Oligonucleotide Therapy for KCNT1 Encephalopathy. JCI Insight, e146090.
35359569	1:1000	Liu, Y., et al. 2022. The Slack Channel Deletion Causes Mechanical Pain Hypersensitivity in Mice. Frontiers in Molecular Neuroscience, 811441.
35359569	1:1000	Liu, Y., et al. 2022. The Slack Channel Deletion Causes Mechanical Pain Hypersensitivity in Mice. Frontiers in Molecular Neuroscience, 811441.
35197318	1:1000	Zhang, Q., et al. 2022. The Slack Channel Regulates Anxiety-like Behaviors via Basolateral Amygdala Glutamatergic Projections to Ventral Hippocampus. The Journal of Neuroscience, 3049-3064.
30860870	1:500	Pryce, K.D., et al. 2019. Magi-1 scaffolds NaV1.8 and Slack KNa channels in dorsal root ganglion neurons regulating excitability and pain. FASEB, 7315-7330.
28982974	not listed	Gururaj, S., et al. 2017. Protein kinase A-induced internalization of Slack channels from the neuronal membrane occurs by adaptor protein-2/clathrin-mediated endocytosis. Journal of Biological Chemistry, 19304-19314.
28943756	1:1000	Tomasello, D.L., et al. 2017. Slick (Kcnt2) Sodium-Activated Potassium Channels Limit Peptidergic Nociceptor Excitability and Hyperalgesia. Journal of Experimental Neuroscience, .
26587966	1:3000	Rizzi, S., et al. 2016. Differential distribution of the sodium-activated potassium channels slick and slack in mouse brain.. Journal of Comparative Neurology, 2093-2116.
29124216	1:3000	Rizzi, S., et al. 2015. Identification of potential novel interaction partners of the sodium-activated potassium channels Slick and Slack in mouse brain. Biochemistry and Biophysics Reports, 291-298.
26559620	not listed	Martinez-Espinosa, P.L., et al. 2015. Knockout of Slo2.2 enhances itch, abolishes KNa current, and increases action potential firing frequency in DRG neurons.. Elife, e10013.
25609627	1:500	Lu, R., et al. 2015. Slack channels expressed in sensory neurons control neuropathic pain in mice.. Journal of Neuroscience, 1125-1135.
22145034	not listed	Wojtovich, A.P., et al. 2011. SLO-2 is cytoprotective and contributes to mitochondrial potassium transport.. PLoS One, e28287.
26845140	not listed	Wojtovich, A.P., et al. 2016. Cardiac Slo2.1 Is Required for Volatile Anesthetic Stimulation of K+ Transport and Anesthetic Preconditioning.. Anesthesiology, 1065-1076.

Western Blot: Rat
PMID	Dilution	Publication
28943756	1:1000	Tomasello, D.L., et al. 2017. Slick (Kcnt2) Sodium-Activated Potassium Channels Limit Peptidergic Nociceptor Excitability and Hyperalgesia. Journal of Experimental Neuroscience, .
28366665	1:2000	Evely, K.M., et al. 2017. The Phe932Ile mutation in KCNT1 channels associated with severe epilepsy, delayed myelination and leukoencephalopathy produces a loss-of-function channel phenotype. Neuroscience, 65-70.
27091544	1:500	Bansal, V., et al. 2016. Na(+) -Activated K(+) Channels in Rat Supraoptic Neurones. Journal of Neuroendocrinology, .
26721627	not listed	Gururaj, S., et al. 2016. Slack sodium-activated potassium channel membrane expression requires p38 mitogen-activated protein kinase phosphorylation.. Neuropharmacology, 279-289.
23872594	1:1000	Huang, F., et al. 2013. TMEM16C facilitates Na(+)-activated K+ currents in rat sensory neurons and regulates pain processing.. Nature Neuroscience, 1284-1290.
20962237	not listed	Nuwer, M.O., et al. 2010. PKA-induced internalization of slack KNa channels produces dorsal root ganglion neuron hyperexcitability.. Journal of Neuroscience, 14165-14172.

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Anti-Slo2.2/Slack K+ Channel Antibody (N3/26)

SKU: 75-051

Product Details

Product Specific References for Applications and Species

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