Products: Primary Antibodies

Anti-Slo2.2/Slack K+ channel Antibody (N3/26)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-051	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $335.00
Brand	Catalog # 73-051	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $335.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC WB

Target Description:

Potassium channel subfamily T member 1 or -KCNT1/Slo2.2/Slack K+ channel is encoded by the gene KCNT1. Slo2.2 is a member of the the voltage-gated ion channel (vic) superfamily, and the KCNT protein family, which is made of KCNT1 and KCNT2. Slo2.2 functions as an outwardly rectifying potassium channel subunit to contribute to ion conductance and signaling pathways. Slo2.2 can be activated by high intracellular sodium or chloride levels. Slo2.2 is expressed at the membrane in many areas of the brain, brainstem, spinal cord, spleen and liver. Mutations in this gene cause the early-onset epileptic disorders

Immunogen:

Fusion protein amino acids 1168-1237 of rat Slo2.2 (Slack; NP_068625); Human: 94% identity (66/70 amino acids identical); Mouse: 98% identity (69/70 amino acids identical)

Cross Reactivity:

No cross-reactivity reported

Protein Name(s):

Slo2.2/Slack K+ channel

Gene Name(s):

Kcnt1 Slack

Antibody Registry ID:

Purified: AB_2131855
TC Supernatant: AB_10698032

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

140 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.