Products: Primary Antibodies

Anti-Slo1/BKAlpha potassium channel (L6/48)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-421	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-421	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Fusion protein amino acids 690-1196 (cytoplasmic C-terminus) of mouse Slo1 (also known as Calcium-activated potassium channel subunit alpha-1, Calcium-activated potassium channel, subfamily M subunit alpha-1, KCa1.1, K(VCA)alpha, BK channel, BKCA alpha, Maxi K channel, Slo-alpha, Slowpoke homolog, Kcnma1 and Kcnma, accession number Q08460)
Rat: 99% identity (448/450 amino acids identical)
Human: 98% identity (446/454 amino acids identical)

Protein Name(s):

Calcium-activated potassium channel subunit alpha-1 (BK channel) (BKCA alpha) (Calcium-activated potassium channel, subfamily M subunit alpha-1) (K(VCA)alpha) (KCa1.1) (Maxi K channel) (MaxiK) (Slo-alpha) (Slo1) (mSlo1) (Slowpoke homolog) (Slo homolog) (mSlo)

Gene Name(s):

Kcnma1 Kcnma

Antibody Registry ID:

Purified: AB_2493097
TC Supernatant: AB_2493095

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

110-130 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Hirono M1, Ogawa Y2, Misono K3, Zollinger DR2, Trimmer JS4, Rasband MN5, Misonou H6.. (2015), 'BK Channels Localize to the Paranodal Junction and Regulate Action Potentials in Myelinated Axons of Cerebellar Purkinje Cells..' J Neurosci. 10.1523/JNEUROSCI.3778-14.2015..
Sailer CA1, Kaufmann WA, Kogler M, Chen L, Sausbier U, Ottersen OP, Ruth P, Shipston MJ, Knaus HG.. (2006), 'Immunolocalization of BK channels in hippocampal pyramidal neurons..' Eur J Neurosci. 10.1111/j.1460-9568.2006.04936.x.