Products: Primary Antibodies

Anti-Shank1 and Shank3 Antibody (N367/51)

from NeuroMab

Product Summary:

This antibody recognizes an epitope found in SH3 and multiple ankyrin repeat domains protein 1 (Shank 1) and SH3 and multiple ankyrin repeat domains protein 3 (Shank3).

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-343	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-343	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Mouse Rat

Applications:

ICC IHC WB

Target Description:

Shank proteins act as scaffold proteins anchoring and connecting membrane bound receptors and other proteins to the cytoskeleton in neurons to enable cell signaling.

Immunogen:

Fusion protein amino acids 538-626 (SH3 domain) of rat Shank3 (also known as SH3 and multiple ankyrin repeat domains protein 3, Proline-rich synapse-associated protein 2, ProSAP2, PSAP2, SPANK-2 and Kiaa1650, accession number Q9JLU4) Mouse: 100% identity (89/89 amino acids identical)
Human: 97% identity (87/89 amino acids identical)
~70% identity with Shank1 and Shank2

Cross Reactivity:

Cross-reacts with Shank1 Does not cross-react with Shank2

Protein Name(s):

SH3 and multiple ankyrin repeat domains protein 1 (Shank1) (GKAP/SAPAP-interacting protein) (SPANK-1) (Somatostatin receptor-interacting protein) (SSTR-interacting protein) (SSTRIP) (Synamon) SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated protein 2) (ProSAP2) (SPANK-2)

Gene Name(s):

Shank1 Shank3 Prosap2

Antibody Registry ID:

Purified: AB_2315919
TC Supernatant: AB_2315918

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

190 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.