Products: Primary Antibodies

Anti-S100A5 Antibody (N176/35)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-199
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-199
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC WB

Target Description:

Protein S100A5 is encoded by the gene S100A5 and a member of the S100 family of proteins. S100A5 is a calcium-binding cytosolic proteins and is also a member of the damage-associated molecular pattern molecules (DAMPs) family, a group of proteins that are released from the extracellular or intracellular space following tissue injury or cell death. S100A5 is localized in the cytoplasm and implicated in the regulation of a number of cellular processes such as cell cycle progression and extracellular signal transduction. S100A5 has been found in brain and kidney. Diseases associated with this gene include Astrocytoma.

Immunogen:

Fusion protein amino acids 1-93 (full-length) of rat S100A (also known as S100 calcium-binding protein A5 and S-100D, accession number P63083); Mouse: 100% identity (93/93 amino acids identical); Human: 93% identity (87/93 amino acids identical); ~50% identity with S100A4 and S100A6

Cross Reactivity:

Does not cross-react with S100A4 or S100A6 (based on KO validation results)

Protein Name(s):

S100A5

Gene Name(s):

S100a5 S100d

Antibody Registry ID:

Purified: AB_2315917
TC Supernatant: AB_2183799


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

10 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.