Products: Primary Antibodies

Anti-RGS14 Antibody (N133/21)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

★ Trial Size Available ★
Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-170
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$325.00
Brand
NeuroMab Logo
Catalog #
75-170-020
Option
Purified Trial-Size
Volume
20 µL
Concentration
1 mg/mL
Price
$109.00
Brand
NeuroMab Logo
Catalog #
73-170
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$325.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Target Description:

RGS14 is a member of the RGS family (regulator of G-protein signaling) and is highly expressed in the prefrontal cortex. Hallmark domains of RGS14 include a RGS domain, two Raf-like Ras-binding domains and a GoLoco motif. RGS14 inactivates G protein a subunits. Alterations in this protein have been implicated in Schizophrenia and Alzheimer’s disease.

Immunogen:

Fusion protein amino acids 1-544 (full-length) of rat RGS14 (also known as Regulator of G-protein signaling 14, RAP1/RAP2-interacting protein and RPIP1, accession number O08773); Mouse: 95% identity (524/547 amino acids identical); Human: 86% identity (475/547 amino acids identical); 45% identity with RGS12 and other RGS proteins; Epitope mapped to within amino acids 444-490 (between 2nd Ras-binding ; GoLoco domains, PDAKTREASSIPPCRSQGCLPRTQTKDSHLPPLSSSLSVEDASGSTG, 2014 Evans et al J Comp Neurol); Mouse: 85% identity (40/47 amino acids identical); Human: 51% identity (24/47 amino acids identical)

Cross Reactivity:

Does not react with RGS12

Protein Name(s):

RGS14

Gene Name(s):

Rgs14

Antibody Registry ID:

Purified: AB_2179931
Purified Trial-Size: AB_2179931
TC Supernatant: AB_10698026


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

60 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.