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Products: Primary Antibodies

Anti-RGS14 (N133/21)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
75-170
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$319.00
Catalog #
75-170-020
Option
Purified Trial-Size
Volume
20 µL
Concentration
1 mg/mL
Price
$99.00
Catalog #
73-170
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$319.00

Validation Images


Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Immunogen:

Fusion protein amino acids 1-544 (full-length) of rat RGS14 (also known as Regulator of G-protein signaling 14, RAP1/RAP2-interacting protein and RPIP1, accession number O08773)
Mouse: 95% identity (524/547 amino acids identical)
Human: 86% identity (475/547 amino acids identical)
45% identity with RGS12 and other RGS proteins
Epitope mapped to within amino acids 444-490 (between 2nd Ras-binding
GoLoco domains, PDAKTREASSIPPCRSQGCLPRTQTKDSHLPPLSSSLSVEDASGSTG, 2014 Evans et al J Comp Neurol)
Mouse: 85% identity (40/47 amino acids identical)
Human: 51% identity (24/47 amino acids identical)

Cross Reactivity:

Does not react with RGS12

Protein Name(s):

Regulator of G-protein signaling 14 (RGS14)

Gene Name(s):

Rgs14

Antibody Registry ID:

Purified: AB_2179931
Purified Trial-Size: AB_2179931
TC Supernatant: AB_10698026


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

60 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.


Citations and References