Products: Primary Antibodies

Anti-RBM17/SPF45 Antibody (N219/5)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-234	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-234	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

RNA-Binding Motif Protein 17 or Splicing factor 45 is encoded by the gene RBM17. RBM17 is a component of the spliceosome complex and displays catalytic activity during mRNA splicing. RBM17 is involved in alternative splicing, resulting in different isoforms of a gene. RBM is also thought to play a role in DNA repair (Red: Chaouki et al 2006) RBM17 is widely expressed in the nucleus and important for development. Diseases associated with this gene include sickle cell anemia and forms of Ataxia 1.

Immunogen:

Fusion protein amino acids 100-200 of human RBM17 (also known as RNA-binding motif protein 17, Splicing factor 45, 45 kDa-splicing factor, SPF45, accession number Q96I25); Mouse: 99% identity (100/101 amino acids identical); Rat: 99% identity (100/101 amino acids identical)

Cross Reactivity:

No cross-reactivity reported

Protein Name(s):

RBM17/SPF45

Gene Name(s):

RBM17 SPF45

Antibody Registry ID:

Purified: AB_10675301
TC Supernatant: AB_10672987

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

45-52 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.