RadioImmunoPrecipitation Assay (RIPA) Buffer, 5X
RIPA buffer is a lysis buffer used to lyse cells and tissues for RadioImmunoPrecipitation assay (RIPA).
RadioImmunoPrecipitation Assay (RIPA) Buffer, 5X
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Ships: 1-2 business days
Preparation: Dilute this 10X buffer 10 times (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, and store 1X buffer solution at 2-8oC for several weeks.
Application: Lysis: Chill 1X buffer on ice add recommended PMSF, protease inhibitor cocktail and phosphatase inhibitor cocktail according to manufacture protocol.
A. Adherent Cells:Wash cells with cold PBS to remove any traces of medium. Add 0.5 ml of 1X RIPA buffer/10 cm dish. Incubate plate on ice for 5 minutes.
Scrape cells, transfer into mirocentrifuge tube, sonicate briefly. Centrifuge extracts for 10 minutes at 14,000 X g in a cold mirocentrifuge, save supernatant.
B. Non-Adherent Cells:Wash cells pellet with cold PBS, centrifuge discard PBS, add approx. equal to cell pellet volume of RIPA buffer, sonicate briefly. Centrifuge extracts for 10 minutes at14,000 x g in cold mirocentrifuge, save supernatant.
Application: Lysis: Chill 1X buffer on ice add recommended PMSF, protease inhibitor cocktail and phosphatase inhibitor cocktail according to manufacture protocol.
A. Adherent Cells:
B. Non-Adherent Cells: