Products: Primary Antibodies

Anti-PSD-95 (K28/43)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
75-028		 Option
Purified		 Volume
100 µL		 Concentration
1 mg/mL		 Price
$325.00
Catalog #
75-028-020		 Option
Purified Trial-Size		 Volume
20 µL		 Concentration
1 mg/mL		 Price
$109.00
Catalog #
73-028		 Option
TC Supernatant		 Volume
5 mL		 Concentration
Lot dependent		 Price
$325.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC IP WB

Immunogen:

Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (also known as Postsynaptic density protein 95, Disks large homolog 4, Synapse-associated protein 90, DLG4, Dlgh4 and SAP-90, accession number P78352)

Rat: 100% identity (223/223 amino acids identical)
Mouse: 99% identity (221/223 amino acids identical)
65-80% identity with DLG1/SAP97, DLG2/Chapsyn-110 and DLG3/SAP102

Cross Reactivity:

No cross-reactivity against recombinant Chapsyn-110, SAP97 or SAP102

Protein Name(s):

Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)

Gene Name(s):

DLG4 PSD95

Antibody Registry ID:

Purified: AB_2292909
Purified Trial-Size: AB_2292909
TC Supernatant: AB_10698024

Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

95-110 kDa(varies with cell background due to phosphorylation)

Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References