Phospho-Sure RTD Neuronal extraction buffer is optimized for the extraction of phosphorylated proteins from neuronal and other soft tissues types. The buffer extracts the phosphoproteins in a native state without the use of harsh detergents or oxidizers, and it is specially formulated to help maintain phosphoproteins and protect them from degradation better than traditional detergent based extraction buffers.
Western blots of Guinea pig brain stem inferior colliculi samples homogenized in Phospho-Sure RTD (B-100-PS) or Lysis buffer and kept on ice for up to 120 min. Aliquots of the homogenates (30 µg) were run on SDS-PAGE and proteins transferred to blotting membrane. Blots were stripped and incubated with 3 antibodies: CREB-P, SAP/JNK-P and ERK 1/2. Image courtesy of Suneja et al (2006).
Detergents typically present in cell lysis buffers are thought to disrupt organelles and compartments and increase the exposure of soluble phospho-proteins to phosphatases and proteases thereby resulting in uncontrolled dephosphorylation and proteolysis.
Phospho-Sure RTD Neuronal extraction buffer is optimized for the extraction of phosphorylated proteins from neuronal and other soft tissues types. The buffer extracts the phosphoproteins in a native state without the use of harsh detergents or oxidizers, and it is specially formulated to help maintain phosphoproteins and protect them from degradation better than traditional detergent based extraction buffers.
IP, WB
Powder containing no preservatives.
Powder
Phospho-sure
For research use only.
United States
Powdered format can be stored up to 12 months after purchase under cool, dry conditions.
The dry, unopened container should be stored at room temperature in a dry or desiccated location protected from light. Do not store in the refrigerator unless material is in a dry, moisture free environment. Material is hydroscopic so once the seal is broken it should be hydrated and not resealed while dry. Once hydrated, the buffer can be stored at 2-8°C for up to 3 months. Solution can be frozen but clumping may occur upon thawing and is not recommended.
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