Phalloidin:FITC

Name: Phalloidin:FITC
Brand: PhosphoSolutions
SKU: PF7501
Price: 168.0 USD
Availability: InStock

Phalloidin conjugated to FITC via the side chain of amino acid 7.

Fluorescent labeling of actin filaments in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The fixed cells were labeled with phalloidin:FITC (PF7501) at a dilution of 1:500.

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Species Reactivity

Chicken, Fish, Human, Mouse, Rat

Applications

ICC, IHC

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Data Sheet

SKU: PF7501

Volume: 100 µL

Variant

Price:

$168.00

Quantity:

Ships: 1-2 business days

Product Details

Scientific Background

Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F-actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. Fluorescent conjugates of phalloidin are commonly used to label actin filaments for immunocytochemical and immunohistochemical applications. Both rhodamine and fluorescein labeled phalloidin can be used to localize and quantify actin filaments in cells and tissues.

Applications

ICC, IHC

Molecular Weight

1250 kDa

Excitation / Emission

FITC Ex: 495 nm, Em: 516 nm

Species Reactivity

Chicken, Fish, Human, Mouse, Rat

Storage

Store at –20°C. Stable for 1 year.

Physical State

Liquid

Buffer

Phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Dilution Ranges

IHC: 1:500
ICC: 1:500

Conjugate

FITC

Specificity

Phalloidin: FITC can be used for immunocytochemical and immunohistochemical labeling of actin filaments. Phalloidin: FITC binds polymeric and oligomeric actin, but does not bind monomeric actin. The conjugates can be used to label actin filaments in many species, including human, mouse, rat, chicken, and fish.

Country of Origin

U.S.A.

Expiration

Stable for 1 year after date of receipt.

Shipping

Blue Ice

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Phalloidin:FITC

SKU: PF7501

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