Pepsin Reagent (Ready to Use)

Pepsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.



SKU: AR-6543-01

Volume: 15ml
Price:
Sale price$68
Pepsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Ready-to use Pepsin reagent
2-8 °C; DO NOT FREEZE
Ships overnight (domestic), International Priority Shipping
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
  1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or ImmunoBioScience Universal Immuno buffer.
  2. Remove buffer, add 2-4 drops of Pepsin reagent to cover tissue section.
  3. Incubate at 37oC or at 56oC for 5-10 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-10 minutes are sufficient at the above temperatures. In some cases it may require 15 minute.).
  4. Discard Pepsin reagent, wash 3-5 times with buffer.
  5. The tissue sections are ready for further IHC protocol.
NOT RECOMMENDED FOR CYTOKERATIN (clone: AE3) ANTIBODY

Product Specific References

PMID Publication
34048095Millares-Ramirez, EM, et al. 2021. Bronchial angiogenesis in horses with severe asthma and its response to corticosteroids. Journal of Veterinary Internal Medicine, 2026-2034.

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