Products: Primary Antibodies

Anti-Pannexin-2 (N121A/1)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-212	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-212	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Rat

Applications:

IHC WB

Immunogen:

Fusion protein amino acids 11-664 (all but first 10 amino acids, encompassing extracellular and cytoplasmic domains) of rat Pannexin-2(also known as Panx2 and Px2, accession number P60571)
Mouse: 98% identity (645/657 amino acids identical)
Human: 91% identity (564/615 amino acids identical)
<30% identity with Pannexin-1 and Pannexin-3

Cross Reactivity:

Does not cross-react with Pannexin-1 or Pannexin-3

Protein Name(s):

Pannexin-2

Gene Name(s):

Panx2 Px2

Antibody Registry ID:

Purified: AB_10673510
TC Supernatant: AB_10671946

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

70 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Citations and References

Le Vasseur M, Lelowski J, Bechberger JF, Sin WC, Naus CC.. (2014), 'Pannexin 2 protein expression is not restricted to the CNS. .' Frontiers in Neuroscience. 10.3389/fncel.2014.00392.