Products: Primary Antibodies

Anti-Pancortin Antibody (K96/7)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-042	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-042	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with K96/7 (left) or N52A/42 (right) TC supe.$

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

Olfactomedin 1 is encoded by the gene OLFM1. While the exact function of OLFM1 is unknown, its abundant expression in the brain suggests it may have an essential role in nerve tissue. Diseases associated with OLFM1 include Ectopic Pregnancy and Neuroblastoma.

Immunogen:

Synthetic peptide of amino acids 131-150 of human Pancortin (GLESKFKQVEE SHKQHLARQ, also known as Noelin, Neuronal olfactomedin-related ER localized protein, Noe1, Noel, Noel1, Olfactomedin-1, Olfm1, 1B426B and D2Sut1e, accession number Q99784); Mouse: 95% identity (19/20 amino acids); Rat: 95% identity (19/20 amino acids); 100% identity with isoforms Pancortin-2,Pancortin-3 and Pancortin-4; 50% identity with Noelin-2 or Noelin-3

Protein Name(s):

Pancortin

Gene Name(s):

OLFM1 NOE1 NOEL1

Antibody Registry ID:

Purified: AB_2267633
TC Supernatant: AB_10674112

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

55-70 kDa (Pancortin-1 and -3),15-20 kDa (Pancortin-2 and -4)

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.