Products: Primary Antibodies

Anti-Pan-QKI Antibody (N147/6)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-168	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-168	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

QKI5, QKI6, QKI7 and QKI7b are alternatively spliced mRNAs from the QKI1 gene. The proteins are RNA-binding and have roles in the regulation of splicing, export and stability of mRNA. They are involved in myelinization.

Immunogen:

Fusion protein amino acids 1-341 (full-length) of human QKI-5 (also known as Protein quaking, HqkI and Hqk, accession number Q96PU8); Mouse: 100% identity (341/341 amino acids identical); Rat: 99% identity (339/341 amino acids identical); Greater than 90% identity with QKI-6, QKI-7 and QKI-7b

Cross Reactivity:

Cross-reacts with QKI-6, QKI-7 and QKI-7b

Protein Name(s):

Pan-QKI

Gene Name(s):

Qki Qk QKI HKQ Qk1 Qka1

Antibody Registry ID:

Purified: AB_2173149
TC Supernatant: AB_10671658

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

36-38 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.