Products: Primary Antibodies

Anti-Pan-Nav1 sodium channel Antibody (N419/40)

from NeuroMab

Product Summary:

This pan antibody recognizes a sequence from the cytoplasmic loop between repeat III and IV common in human Nav1 channels.

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-405	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $335.00
Brand	Catalog # 73-405	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $335.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot against fractions of adult rat brain membranes (RBM) and extracts of COS or HEK cells transiently or stably expressing different untagged Nav1.X or Kv2.1 plasmid probed with N419/40 (left) or K58/35 (right) TC supe.$

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

The family of voltage gated sodium channels comprise a large alpha subunit and auxiliary beta subunits. Nav1 channels are members of the voltage gated sodium ion channel family and form the alpha type, pore-forming subunit of the channel. There are nine known members of this family. These alpha subunits vary in their function and tissue expression.

Immunogen:

Synthetic peptide amino acids 1501-1518 (TEEQKKYYNAMKKLGSKK, cytoplasmic loop between repeat III and IV) of human Nav1.1 (also known as Voltage-gated sodium channel protein type/brain 1 subunit alpha, SCN1A, NAC1 and SCN1, accession number P35498) Mouse: 100% identity (18/18 amino acids identical)
Rat: 100% identity (18/18 amino acids identical)
100% identity with all Nav1 channels

Cross Reactivity:

Cross-reacts with multiple Nav1 channels

Protein Name(s):

Sodium channel protein type 2 subunit alpha (HBSC II) (Sodium channel protein brain II subunit alpha) (Sodium channel protein type II subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.2) Sodium channel protein type 1 subunit alpha (Sodium channel protein brain I subunit alpha) (Sodium channel protein type I subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.1) Sodium channel protein type 9 subunit alpha (Neuroendocrine sodium channel) (hNE-Na) (Peripheral sodium channel 1) (PN1) (Sodium channel protein type IX subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.7) Sodium channel protein type 5 subunit alpha (HH1) (Sodium channel protein cardiac muscle subunit alpha) (Sodium channel protein type V subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.5) Sodium channel protein type 3 subunit alpha (Sodium channel protein brain III subunit alpha) (Sodium channel protein type III subunit alpha) (Voltage-gated sodium channel subtype III) (Voltage-gated sodium channel subunit alpha Nav1.3) Sodium channel protein type 8 subunit alpha (Sodium channel protein type VIII subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.6) Sodium channel protein type 10 subunit alpha (Peripheral nerve sodium channel 3) (PN3) (hPN3) (Sodium channel protein type X subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.8) Sodium channel protein type 4 subunit alpha (SkM1) (Sodium channel protein skeletal muscle subunit alpha) (Sodium channel protein type IV subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.4) Sodium channel protein type 11 subunit alpha (Peripheral nerve sodium channel 5) (PN5) (Sensory neuron sodium channel 2) (Sodium channel protein type XI subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.9)

Gene Name(s):

SCN2A NAC2 SCN2A1 SCN2A2 SCN1A NAC1 SCN1 SCN9A NENA SCN5A SCN3A KIAA1356 NAC3 SCN8A MED SCN10A SCN4A SCN11A SCN12A SNS2

Antibody Registry ID:

Purified: AB_2491098
TC Supernatant: AB_2491079

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

>200 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.