Products: Primary Antibodies

Anti-Pan-KChIP K+ Channel Antibody (K55/82)

NeuroMab Logo from NeuroMab

 Datasheet

Product Summary:

This pan-kChiP antibody recognizes an epitope common the Kv channel-interacting proteins KChIP1, KChIP2, KChIP3 and KChIP4 and is suitable for IHC, IP, and WB applications.

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-006
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-006
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC IP WB

Target Description:

The Kv channel-interacting proteins (KChIP1, kChIp2, kChIp3, and kChIp4) are members of the family of voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs), which belong to the recoverin or neuronal calcium sensor (NCS) family branch of the EF-hand superfamily. They serve as auxiliary channel subunits and interact with Kv4 alpha subunits to form native Kv4 channel complexes. KChiP subunits regulate Kv4 channels through various mechanisms including altering: channel density, inactivation kinetics, rate of recovery from inactivation, and cell surface delivery.

Immunogen:

Fusion protein amino acids 1-216 (full length) of rat KChIP1b (accession number Q8R426); Human: 99.5% identity (215/216 amino acids); Mouse=100% identity (216/216 amino acids)

Cross Reactivity:

No cross-reactivity reported

Protein Name(s):

Pan-KChIP K+ channel

Gene Name(s):

Kcnip1 Kchip1

Antibody Registry ID:

Purified: AB_10673406
TC Supernatant: AB_2132595


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

33 kDa (KChIP2)25 kDa (KChIP3/4)22 kDa (KChIP1)


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.