Products: Primary Antibodies

Anti-Pan-Gamma-protocadherin-Constant (N159/5)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-185	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-185	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse

Applications:

IHC WB

Immunogen:

Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Gamma-protocadherin-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941)
Human: 99% identity (123/124 amino acids identical)

Cross Reactivity:

Cross-reacts with all Gamma protocadherin-A, -B and -C proteins

Protein Name(s):

Protocadherin gamma A3 (Protocadherin gamma subfamily A, 3)

Gene Name(s):

Pcdhga3

Antibody Registry ID:

Purified: AB_2159449
TC Supernatant: AB_10676098

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

100 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Keeler AB, Schreiner D, Weiner JA.. (2015), 'Protein Kinase C Phosphorylation of a γ-Protocadherin C-terminal Lipid Binding Domain Regulates Focal Adhesion Kinase Inhibition and Dendrite Arborization. .' The Journal of Biological Chemistry. 10.1074/jbc.M115.642306.
de Andrade GB, Kunzelman L, Merrill MM, Fuerst PG.. (2014), 'Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina. .' Molecular Vision. .
Chen WV, Alvarez FJ, Lefebvre JL, Friedman B, Nwakeze C, Geiman E, Smith C, Thu CA, Tapia JC, Tasic B, Sanes JR, Maniatis T.. (2012), 'Functional significance of isoform diversification in the protocadherin gamma gene cluster. .' Neuron. 10.1016/j.neuron.2012.06.039.
Garrett AM, Schreiner D, Lobas MA, Weiner JA.. (2012), 'γ-protocadherins control cortical dendrite arborization by regulating the activity of a FAK/PKC/MARCKS signaling pathway. .' Neuron. 10.1016/j.neuron.2012.01.028.
Prasad T, Weiner JA.. (2011), 'Direct and Indirect Regulation of Spinal Cord Ia Afferent Terminal Formation by the γ-Protocadherins. .' Frontiers in Molecular Neuroscience. 10.3389/fnmol.2011.00054.
Lobas MA, Helsper L, Vernon CG, Schreiner D, Zhang Y, Holtzman MJ, Thedens DR, Weiner JA. (2012), 'Molecular heterogeneity in the choroid plexus epithelium: the 22-member γ-protocadherin family is differentially expressed, apically localized, and implicated in CSF regulation. .' Journal of Neurochemistry. 10.1111/j.1471-4159.2011.07587.x.
O'Leary R, Reilly JE, Hanson HH, Kang S, Lou N, Phillips GR.. (2011), 'A variable cytoplasmic domain segment is necessary for γ-protocadherin trafficking and tubulation in the endosome/lysosome pathway. .' Molecular Biology of the Cell. 10.1091/mbc.E11-04-0283.
Fernández-Monreal M, Oung T, Hanson HH, O'Leary R, Janssen WG, Dolios G, Wang R, Phillips GR.. (2010), 'γ-protocadherins are enriched and transported in specialized vesicles associated with the secretory pathway in neurons. .' European Journal of Neuroscience. 10.1111/j.1460-9568.2010.07386.x.
Li Y, Serwanski DR, Miralles CP, Fiondella CG, Loturco JJ, Rubio ME, De Blas AL.. (2010), 'Synaptic and nonsynaptic localization of protocadherin-gammaC5 in the rat brain. .' Journal of Comparative Neurology. 10.1002/cne.22390.
Garrett AM, Weiner JA.. (2009), 'Control of CNS synapse development by {gamma}-protocadherin-mediated astrocyte-neuron contact. .' Journal of Neuroscience. 10.1523/JNEUROSCI.2818-09.2009.