Products: Primary Antibodies

Anti-Pan-Gamma-protocadherin-Constant Antibody (N159/5)

from NeuroMab

Product Summary:

This antibody recognizes an epitope that is shared by all 22 Gamma-protocadherins (Cross-reacts with all Gammaprotocadherin-A, -B and -C proteins).

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-185	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-185	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse

Applications:

IHC WB

Target Description:

Gamma-protocadherins belong to a superfamily of homophilic cell-adhesion proteins. These cell adhesion proteins are thought to be involved in establishing and function of cell-cell connections in brain. These proteins are widely expressed in the nervous system and brain.

Immunogen:

Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Gamma-protocadherin-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941); Human: 99% identity (123/124 amino acids identical)

Cross Reactivity:

Cross-reacts with all Gamma protocadherin-A, -B and -C proteins

Protein Name(s):

Pan-Gamma-protocadherin-Constant

Gene Name(s):

Pcdhga3

Antibody Registry ID:

Purified: AB_2159449
TC Supernatant: AB_10676098

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

100 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.