Products: Primary Antibodies

Anti-Pan-Gamma-protocadherin-Constant Antibody (N159/5)

NeuroMab Logo from NeuroMab

 Datasheet

Product Summary:

This antibody recognizes an epitope that is shared by all 22 Gamma-protocadherins (Cross-reacts with all Gammaprotocadherin-A, -B and -C proteins).

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-185
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-185
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC WB

Target Description:

Gamma-protocadherins belong to a superfamily of homophilic cell-adhesion proteins. These cell adhesion proteins are thought to be involved in establishing and function of cell-cell connections in brain. These proteins are widely expressed in the nervous system and brain.

Immunogen:

Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Gamma-protocadherin-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941); Human: 99% identity (123/124 amino acids identical)

Cross Reactivity:

Cross-reacts with all Gamma protocadherin-A, -B and -C proteins

Protein Name(s):

Pan-Gamma-protocadherin-Constant

Gene Name(s):

Pcdhga3

Antibody Registry ID:

Purified: AB_2159449
TC Supernatant: AB_10676098


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

100 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.