Anti-NSD1 Antibody (N312/10)

Name: Anti-NSD1 Antibody
Brand: NeuroMab
SKU: 75-280
Price: 374.0 USD
Availability: InStock

Our Anti-NSD1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N312/10. It detects human and mouse NSD1, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged NSD1, NSD2, NSD3 or untagged Kv2.1 plasmids and probed with N312/10 (left), 12CA5 (middle) and K89/34 (right).

Species Reactivity

Human, Mouse

Applications

ICC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

Trial Size

SKU: 75-280

Volume: 100 µL

Variant

Price:

$374.00

Quantity:

Product Details

Target

NSD1

Target Description

Nuclear Receptor Binding SET Domain Protein 1 is encoded by the gene NSD1. NSD1 is a member of the class V-like SAM-binding methyltransferase superfamily. NSD1 is a histone methyltransferase that is capable of both negatively or positively influencing transcription, depending on the cellular context. NSD1 is expressed in the brain, kidney, spleen, skeletal muscle, thymus, and lung. Diseases associated with NSD1 include Sotos Syndrome 1 and Overgrowth Syndrome.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N312/10

Isotype

IgG2b

Applications

ICC, WB

Host Species

Mouse

Gene Name

NSD1 ARA267 KMT3B

Molecular Weight

300 kDa

Antigen

Fusion protein amino acids 2129-2696 (C-terminus) of human NSD1 (accession number Q96L73) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_11001827

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Does not cross-react with NSD2 or NSD3

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific (EC 2.1.1.43) (Androgen receptor coactivator 267 kDa protein) (Androgen receptor-associated protein of 267 kDa) (H3-K36-HMTase) (H4-K20-HMTase) (Lysine N-methyltransferase 3B) (Nuclear receptor-binding SET domain-containing protein 1) (NR-binding SET domain-containing protein)

UniProt Details

UniProt (Human): Q96L73
UniProt (Immunogen Species): Q96L73

Papillon-Cavanagh S1, Lu C2, Gayden T1, Mikael LG3, Bechet D1, Karamboulas C4, Ailles L4, Karamchandani J5, Marchione DM6,7, Garcia BA6, Weinreb I8, Goldstein D9, Lewis PW10, Dancu OM1, Dhaliwal S11,12, Stecho W13, Howlett CJ13, Mymryk JS14,15, Barrett JW11,12,15, Nichols AC11,12,15, Allis CD2, Majewski J1, Jabado N1,3. (2017), 'Impaired H3K36 methylation defines a subset of head and neck squamous cell carcinomas..' Nat Genet. 10.1038/ng.3757.
Satterlee JS1, Beckel-Mitchener A, McAllister K, Procaccini DC, Rutter JL, Tyson FL, Chadwick LH. (2015), 'Community resources and technologies developed through the NIH Roadmap Epigenomics Program..' Methods Mol Biol. 10.1007/978-1-4939-1804-1_2.

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Anti-NSD1 Antibody (N312/10)

SKU: 75-280

Product Details

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