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Products: Primary Antibodies

Anti-Npas4 (N408/79)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
75-396
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$269.00
Catalog #
73-396
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$269.00

Validation Images


Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Immunogen:

Fusion protein amino acids 597-802 (C-terminus) of rat Npas4 (also known as Neuronal PAS domain-containing protein 4, Neuronal PAS4, Class E basic helix-loop-helix protein 79, HLHPAS transcription factor NXF, PAS domain-containing protein 10, Limbic-enhanced PAS protein, bHLHe79, PASD10 and LE-PAS, accession number Q8CJH6) Mouse: 99% identity (205/206 amino acids identical)
Human: 93% identity (193/206 amino acids identical)
25% identity with other PAS domain-containing proteins

Protein Name(s):

Neuronal PAS domain-containing protein 4 (Neuronal PAS4) (HLH-PAS transcription factor NXF)

Gene Name(s):

Npas4 Nxf

Antibody Registry ID:

Purified: AB_2491085
TC Supernatant: AB_2336912


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

90 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.