Products: Primary Antibodies

Anti-Npas4 (N408/79)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-396	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-396	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Fusion protein amino acids 597-802 (C-terminus) of rat Npas4 (also known as Neuronal PAS domain-containing protein 4, Neuronal PAS4, Class E basic helix-loop-helix protein 79, HLHPAS transcription factor NXF, PAS domain-containing protein 10, Limbic-enhanced PAS protein, bHLHe79, PASD10 and LE-PAS, accession number Q8CJH6) Mouse: 99% identity (205/206 amino acids identical)
Human: 93% identity (193/206 amino acids identical)
25% identity with other PAS domain-containing proteins

Protein Name(s):

Neuronal PAS domain-containing protein 4 (Neuronal PAS4) (HLH-PAS transcription factor NXF)

Gene Name(s):

Npas4 Nxf

Antibody Registry ID:

Purified: AB_2491085
TC Supernatant: AB_2336912

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

90 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.