Non ionic detergents like NP40 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction.
Non ionic detergents like NP40 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from 0.1- 0.5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background. Tergitol-type NP-40, which is nonyl phenoxylpolyethoxylethanol, lower surface tension of water.
ELISA, IHC
This detergent is supplied as 25% in water with 0.15 % preservative.
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
Room Temperature
Ships overnight (domestic), International Priority Shipping
For diluting antibodies use 1:1000 in buffers (1 ml into 999 ml).
Washing buffer use 1:500 (1 ml into 499 ml).
The optimum dilution should be determined by the individual lab. (NOT RECOMMENDED FOR DILUTION OF S100 ANTIBODIES)
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