Products: Primary Antibodies

Anti-NKCC1 (N161/20)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
75-229		 Option
Purified		 Volume
100 µL		 Concentration
1 mg/mL		 Price
$275.00
Catalog #
73-229		 Option
TC Supernatant		 Volume
5 mL		 Concentration
Lot dependent		 Price
$275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

DB

Immunogen:

Synthetic peptide amino acids 208-223 (YYLR[pT]FGHN[pT]MDAVPR) of human NKCC1 (also known as Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, Solute carrier family 12 member 2 and Slc12a2, accession number P55011)
Mouse: 100% identity (16/16 amino acids identical)
Rat: 100% identity (16/16 amino acids identical)
>90% identity with NKCC2
>70% identity with NKCC3

Cross Reactivity:

Cross-reacts with non-phosphorylated protein by ELISA

Protein Name(s):

Solute carrier family 12 member 2 (Basolateral Na-K-Cl symporter) (Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1)

Gene Name(s):

SLC12A2 NKCC1

Antibody Registry ID:

Purified: AB_2285991
TC Supernatant: AB_10675296

Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Molecular Weight:

None Reported

Citations and References