Products: Primary Antibodies

Anti-NKCC1 (N161/20)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-229	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-229	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

Immunogen:

Synthetic peptide amino acids 208-223 (YYLR[pT]FGHN[pT]MDAVPR) of human NKCC1 (also known as Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, Solute carrier family 12 member 2 and Slc12a2, accession number P55011)
Mouse: 100% identity (16/16 amino acids identical)
Rat: 100% identity (16/16 amino acids identical)
>90% identity with NKCC2
>70% identity with NKCC3

Cross Reactivity:

Cross-reacts with non-phosphorylated protein by ELISA

Protein Name(s):

Solute carrier family 12 member 2 (Basolateral Na-K-Cl symporter) (Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1)

Gene Name(s):

SLC12A2 NKCC1

Antibody Registry ID:

Purified: AB_2285991
TC Supernatant: AB_10675296

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Expected Banding Pattern:

None Reported

Citations and References

Campbell SL, Robel S, Cuddapah VA, Robert S, Buckingham SC, Kahle KT, Sontheimer H.. (2015), 'GABAergic disinhibition and impaired KCC2 cotransporter activity underlie tumor-associated epilepsy. .' Glia. 10.1002/glia.22730.
Antoine MW, Hübner CA, Arezzo JC, Hébert JM.. (2013), 'A causative link between inner ear defects and long-term striatal dysfunction. .' Science. 10.1126/science.1240405.