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Products: Primary Antibodies

Anti-NKCC1 Antibody (N161/20)

NeuroMab Logo from NeuroMab

 Datasheet

Product Summary:

This antibody was raised against a phosphorylated sequence of the protein for NKCC1.

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo		 Catalog #
75-229		 Option
Purified		 Volume
100 µL		 Concentration
1 mg/mL		 Price
$275.00
Brand
NeuroMab Logo		 Catalog #
73-229		 Option
TC Supernatant		 Volume
5 mL		 Concentration
Lot dependent		 Price
$275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

DB

Target Description:

NKCC1 or Solute carrier family 12 member 2, is encoded by the gene SLC12A2. NKCC1 is a membrane bound protein that is involved in sodium, potassium and chloride transport and reabsorption and is expressed in many tissues.

Immunogen:

Synthetic peptide amino acids 208-223 (YYLR[pT]FGHN[pT]MDAVPR) of human NKCC1 (also known as Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, Solute carrier family 12 member 2 and Slc12a2, accession number P55011); Mouse: 100% identity (16/16 amino acids identical); Rat: 100% identity (16/16 amino acids identical); Greater than 90% identity with NKCC2; Greater than 70% identity with NKCC3

Cross Reactivity:

Cross-reacts with non-phosphorylated protein by ELISA

Protein Name(s):

NKCC1

Gene Name(s):

SLC12A2 NKCC1

Antibody Registry ID:

Purified: AB_2285991
TC Supernatant: AB_10675296

Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Molecular Weight:

None Reported