Products: Primary Antibodies

Anti-NGL-2/LRRC4 (N50/36)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-084	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-084	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 550-652 of mouse NGL-2 (accession number AAG60620)
Human: 98% identity (101/103 amino acids identical)
Rat: 99% identity (102/103 amino acids identical)

Cross Reactivity:

No cross-reactivity against NGL-1 or NGL-3

Protein Name(s):

Leucine-rich repeat-containing protein 4 (Brain tumor-associated protein MBAG1) (Netrin-G2 ligand) (NGL-2)

Gene Name(s):

Lrrc4

Antibody Registry ID:

Purified: AB_2137614
TC Supernatant: AB_10672415

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

100 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Soto F, Watkins KL, Johnson RE, Schottler F, Kerschensteiner D.. (2013), 'NGL-2 regulates pathway-specific neurite growth and lamination, synapse formation, and signal transmission in the retina. .' Journal of Neuroscience. 10.1523/JNEUROSCI.1521-13.2013.
DeNardo LA, de Wit J, Otto-Hitt S, Ghosh A.. (2012), 'NGL-2 regulates input-specific synapse development in CA1 pyramidal neurons. .' Neuron. 10.1016/j.neuron.2012.10.013.
Zhang W, Rajan I, Savelieva KV, Wang CY, Vogel P, Kelly M, Xu N, Hasson B, Jarman W, Lanthorn TH.. (2008), 'Netrin-G2 and netrin-G2 ligand are both required for normal auditory responsiveness. .' Genes, Brain and Behavior. 10.1111/j.1601-183X.2007.00361.x.