Fusion protein amino acids 550-652 of mouse NGL-2 (accession number AAG60620)
Human: 98% identity (101/103 amino acids identical)
Rat: 99% identity (102/103 amino acids identical)
No cross-reactivity against NGL-1 or NGL-3
Leucine-rich repeat-containing protein 4 (Brain tumor-associated protein MBAG1) (Netrin-G2 ligand) (NGL-2)
TC Supernatant: AB_10672415
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
Citations and References
- Soto F, Watkins KL, Johnson RE, Schottler F, Kerschensteiner D.. (2013), 'NGL-2 regulates pathway-specific neurite growth and lamination, synapse formation, and signal transmission in the retina. .' Journal of Neuroscience. 10.1523/JNEUROSCI.1521-13.2013. " "
- DeNardo LA, de Wit J, Otto-Hitt S, Ghosh A.. (2012), 'NGL-2 regulates input-specific synapse development in CA1 pyramidal neurons. .' Neuron. 10.1016/j.neuron.2012.10.013. " "
- Zhang W, Rajan I, Savelieva KV, Wang CY, Vogel P, Kelly M, Xu N, Hasson B, Jarman W, Lanthorn TH.. (2008), 'Netrin-G2 and netrin-G2 ligand are both required for normal auditory responsiveness. .' Genes, Brain and Behavior. 10.1111/j.1601-183X.2007.00361.x. "