Neurotrophins are growth factors that regulate neuronal development, maintenance and plasticity. These homo-dimeric proteins activate a multitude of intracellular signalling cascades through their interaction with Trk receptors and p75NTR.
The Neurotrophin Antibody Kit (Trial Size) contains a collection of Biosensis' popular sheep antibodies raised against BDNF (S-015-500), NGF (S-052-500), NT3 (S-057-500) and NT4/5 (S-059-500). The kit is completed with one vial of normal sheep IgG (S-1754-100) for use as negative control. This trial size of Biosensis' Neurotrophin Antibody Kit contains 100 µg sheep IgG of each antibody.
This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.
Protein G purified IgG
Polyclonal
IgG
IHC, Neutralize
Sheep
BDNF: recombinant human BDNF NGF: native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE) NT3: recombinant human NT3 NT4/5: recombinant human NT4
Human
Human, Mouse, Rat
Spin vial briefly before opening. Reconstitute each vial with 100 µL sterile-filtered, ultrapure water, to achieve an antibody concentration of 1 mg/mL. Centrifuge briefly to remove any insoluble material. Store lyophilized, unopened vials at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Lyophilized
Lyophilized from PBS, pH 7.4, without preservatives.
WB: 1-10 µg/mL
IHC: 1-10 µg/mL
ELISA: 1-10 µg/mL
Immunohistochemistry: 1-10 µg/mL. BDNF antibody works best on Zamboni's fixed, frozen tissue, and is not recommended for paraffin-embedded tissue. ELISA: 1-10 µg/mL. Western Blotting: 1-10 µg/mL. Western blotting is not recommended for the BDNF antibody. Inhibition of biological activity in vitro/in vivo: Expected ED50 (in vivo) values are in the range of 2-10 µg/mL. The NGF antibody completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. The negative control (normal sheep IgG) should be used at the same concentration as the neurotrophin antibodies.
Unconjugated
BDNF Antibody: A cross-reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by ELISA. NGF Antibody: A cross-reactivity of less than 1% against recombinant human BDNF, NT3 and NT4/5 by ELISA has been shown. NT3 Antibody: A cross-reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by ELISA. NT4/5 Antibody: Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Neurotrophins share a large degree of homology among human, rodent and other mammalian species, thus these neurotrophin antibodies are useful for a number of species, including human, mouse and rat.
For research use only.
United States
Store at 2-8°C for up to 3 months. For longer storage, aliquot and store at -20°C for up to 12 months.
