Products: Primary Antibodies

Anti-Navbeta1 Na+ channel Antibody (N405/74)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-425	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-425	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Mouse Rat

Applications:

ICC IHC

Target Description:

Werner syndrome ATP-dependent helicase, WRN RecQ Like Helicase or DNA Helicase, RecQ-Like Type 3 is encoded by the gene WRN and is a member of the WRN RecQ Like Helicase family. WRN acts as a DNA helicase that is involved in DNA repair, replication, recombination and maintenance of DNA stability. WRN is a multifunctional enzyme with ATP dependent helicase activity, and is the only RecQ helicase member that has 3’ to 5’ exonuclease activity. WRN is expressed within the central nervous system and brain Diseases associated with this gene include Werner Syndrome and Medulloblastoma.

Immunogen:

Fusion protein amino acids 14-160 (extracellular N-terminus) of rat Navbeta1 (also known as Sodium channel subunit beta-1 and Scn1b, accession number Q00954) Mouse: 99% identity (146/147 amino acids identical)
Human: 97% identity (143/147 amino acids identical)
50% identity with Navbeta2 or Navbeta3

Cross Reactivity:

Does not cross-react with Navbeta2, Navbeta3 or Navbeta4

Protein Name(s):

Sodium channel subunit beta-1

Gene Name(s):

Scn1b

Antibody Registry ID:

Purified: AB_2637012
TC Supernatant: AB_2531891

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.