Mouse NGFR/p75ECD Rapid ELISA Assay

Name: Mouse NGFR/p75ECD Rapid ELISA Assay
Brand: Biosensis
SKU: BEK-2220-2P
Price: 787.0 USD
Availability: InStock

This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.

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Species Reactivity

Mouse

Applications

ELISA

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Data Sheet

SKU: BEK-2220-2P

Volume/Size: 2 Plate Kit

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Product Details

Product Description

The Biosensis NGFR/p75^ECD Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse p75^ECD in less than 4 hours in cell culture supernatants and urine only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.

This ELISA kit consists of a pre-coated mouse monoclonal anti-p75^ECD capture antibody, a goat anti- p75^ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75^ECD present in samples and protein standards.

This NGFR/p75^ECD ELISA kit employs a recombinant mouse p75^ECD-Fc chimera protein as standard. While there is a current lack of a true mouse p75^ECD standard, this ELISA kit allows quantification of mouse p75^ECD as p75^ECD-Fc mouse equivalents. Please note that the antibodies used in this ELISA cross-react with human NGFR/p75^ECD.

This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.

Scientific Background

The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75^ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75^ECD levels are found in urine of ALS patients as compared to healthy controls.

Target

Nerve growth factor receptor, extracellular domain (NGFR/p75ECD)

Immunogen

Mouse myeloma cell line, NS0-derived mouse p75 (AA 20-243) -Fc chimera

Immunogen Species

Mouse

Range

62.5 - 4,000 pg/mL

Sensitivity

This ELISA kit typically detects 20-40 pg/mL of mouse p75ECD (defined as blank OD plus 3x the standard deviation of the blank OD, n=10).

Specificity

Mouse. The antibodies used in this ELISA kit are known to cross-react with human p75^ECD protein.

Species Reactivity

Mouse

Applications

ELISA

Method of Analysis

Colorimetric

Sample Type

Culture Supernatant, Tissue Homogenates, Urine

Synonyms

Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; Tumor necrosis factor receptor superfamily member 16; CD271; NGFR; TNFRSF16; NGFR/p75ECD; p75ECD

UniProt ID

Q9Z0W1

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months from purchase.

Storage

Store at 2-8°C.

Shipping

Shipped on ice packs

Mouse NGFR/p75ECD Rapid ELISA Assay Protocol
Technical Note 1 - ELISA Validation
Technical Note 2 - ELISA Data Analysis
Calculation of Antigen Amount in Tissues and Lysates

Reagent	1 Plate Kit	2 Plate Kit
p75^ECD antibody coated 96 well microplate	12 × 8 wells	24 × 8 wells
Assay diluent A (1X)	2 × 25 mL	4 × 25 mL
Recombinant mouse p75^ECD-Fc standard	2 × 8 ng	4 × 8 ng
Quality Control (QC) Sample	2 Vials (see vial label for amount)	4 Vials (see vial label for amount)
Lyophilized p75^ECD detection antibody (100X)	1 Vial	2 Vials
Anti-goat HRP conjugate (100X)	1 × 110 µL	2 × 110 µL
Wash buffer (10X)	1 × 33 mL	2 × 33 mL
TMB substrate (1X)	1 × 11 mL	2 × 11 mL
TMB stop solution (1X)	1 × 11 mL	2 × 11 mL
Plate sealer	Supplied

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Mouse NGFR/p75ECD Rapid ELISA Assay

SKU: BEK-2220-2P

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