Methylene Blue Counter Stain
This methylene Blue counterstain solution is designed for nuclear counterstain after completion of Immunohistochemistry staining or for routine histology.
Methylene Blue Counter Stain
Click on image to zoom
Ships: 1-2 business days
A. Immunohistochemistry:
- After chromogen staining, wash slide 2-3X with de/di water, 2X with buffer and 2X with de/di water
- Apply Methylene Blue counterstain for 3-5 minutes or longer, if darker color is required.
- Rinse with water, followed by alcohol gradients 30 to 95%; 100% alcohol 5-7X.
- Clear with Xylene or clearing agent.
- The slides can be mounted with Organic mounting mediums like Organo mount (limonene) or equivalent.
- Deparafinize formalin-fixed paraffin-embedded (FFPE) tissue sections.
- At the counterstain step, apply Methylene Blue counterstain for 3-5 minutes or longer if darker color is required.
- Rinse with water, dehydrate with gradients of alcool 30-95%; and 100% alcohol 5-7X.
- Clear with Xylene or clearing agent.
- The slides can be mounted with Organic mounting mediums like Organo mount (ImmunoBioscience cat. # AR-6504) or equivalent.