LipoFluor-P1™ Polar Lipid Tracing Reagent

Name: LipoFluor-P1™ Polar Lipid Tracing Reagent
Brand: Biosensis
SKU: TR-600-P1
Price: 297.0 USD
Availability: InStock

Staining of lipids with TR-600-P1 in muscle tissue (A), cardiomyocytes (B), vesicular structures (C) and fenal adipose tissue (D).

Staining of lipids with TR-600-P1 in adipocytes (E).

Staining of lipids with TR-600-P1 in muscle tissue (A), cardiomyocytes (B), vesicular structures (C) and fenal adipose tissue (D).

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Species Reactivity

All Mammals, Human, Mouse

Applications

ICC, IF

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Data Sheet

SKU: TR-600-P1

Volume/Size: 4 vials x 5.5 µL per vial

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$297.00

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Product Details

Product Description

LipoFluor-P1™ is a cell-permeant stain with an affinity for polar lipids which can be used in a wide variety of live and fixed samples. LipoFluor-P1™ provides excellent and rapid lipid staining used for fluorescent microscopy applications and automated imaging. This stain is an effective tracer of lipids (e.g. cholesterol, sphingolipid and phospholipid) and intracellular localisation. This stain allows for simple and quick application and is ideal for epifluorescence, confocal and multiphoton microscopy. It can be used on live and fixed samples and is compatible with other dyes. LipoFluor-P1™ has a large Stokes Shift, is resistant to photobleaching and demonstrates low cytotoxicity.

Target

Polar Lipids

Immunogen Species

Human

Specificity

Fluorescent imaging of lipid-rich compartments. LipoFluor-P1™ is an effective tracer of lipids (e.g., cholesterol, sphingolipid, and phospholipid) and intracellular localization. Cell penetration and localisation of LipoFluor-P1™ has been confirmed in a range of cell lines; including adipocytes (3T3-L1), prostate cells (PNT2, PNT1a, LNCaP, 22RV1 and DU145), cardiomyocytes (H9c2) and neuronal cells (PC-12), and tissues; including adipose tissue (sheep and Drosophila), muscle tissue (sheep cardiac and skeletal) and brain (murine).

Species Reactivity

All Mammals, Human, Mouse

Applications

ICC, IF

Application Details

Fluorescent imaging of lipid-rich compartments. Sample types: live cells, fixed cells (4% paraformaldehyde), tissue (frozen only); Microscopy types: Epifluorescence microscopy: YES, Confocal microscopy: YES, Multiphoton imaging: YES, Infrared spectroscopy: YES, Raman spectroscopy: YES. Visualization: Ex/Em: 405 nm / 550 nm. Use instruction: The typical working concentration is 10µM (1:1000 dilution) in an appropriate buffer or cell culture medium. Biosensis recommends that the end user determine optimal dilutions/concentrations.

Excitation / Emission

405 nm / 550 nm

Method of Analysis

Fluorescence

Sample Type

Cells, Tissues (live or fixed)

Buffer

DMSO

Physical State

Liquid, ready to dilute

Production Notes

Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of fluorescent isomers. No amount of starting material was detected.

Synonyms

LipoFluor-P1™

Usage Statement

For research use only.

Country of Origin

United States

Expiration

If protected from light, the liquid 10 mM stock solution vials are stable for 12 months from date of receipt, at 2-8ºC. A vial should be diluted immediately prior to use and should be used within 24 hours for best results.

Storage

The liquid stock solution vials can be stored up to 12 months from date of receipt at 2-8°C, properly sealed and protected from light. Working solutions should be used within 24 hours and not stored for later use.

Shipping

25°C (ambient)

LipoFluor-P1™ Protocol

Four 0.5 mL microfuge vials containing 5.5 uL/vial of 10 mM stock solution of Lipofluor-P1™ TR-600-P1 in DMSO. 34.1 μg per vial.

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LipoFluor-P1™ Polar Lipid Tracing Reagent

SKU: TR-600-P1

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