LipoFluor-MR™ Mitochondria Tracing Reagent

Name: LipoFluor-MR™ Mitochondria Tracing Reagent
Brand: Biosensis
SKU: TR-602-Mito
Price: 297.0 USD
Availability: InStock

LipoFluor-MR™ is a cell-permeant stain for mitochondria. This tracing reagent can be used on live cells, frozen and fixed tissue samples and is compatible with other dyes.

Staining of mitochondria in cardiomyocytes (top) and HeLa cells (bottom) with TR-602-Mito tracing reagent.

Staining of mitochondria in cardiomyocytes (top) and HeLa cells (bottom) with TR-602-Mito tracing reagent.

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Species Reactivity

All Mammals, Human, Mouse

Applications

ICC, IF

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Data Sheet

SKU: TR-602-Mito

Volume/Size: 4 vials x 5.5 µL per vial

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$297.00

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Product Description

LipoFluor-MR™ is a cell-permeant stain for mitochondria. This tracing reagent can be used on live cells, frozen and fixed tissue samples and is compatible with other dyes. LipoFluor-MR™ has a large Stokes Shift, shows excellent resistance to photobleaching and demonstrates low cytotoxicity.

Target

Mitochondria

Immunogen Species

Human

Specificity

Fluorescent imaging of mitochondria. Cell penetration and localisation of LipoFluor-MR™ has been confirmed in prostate cells (PNT2 and 22RV1), cardiomyocytes (H9c2) and cancer cell lines (HeLa). Mitochondrial localisation has been shown in live tissues, including live adipose tissue (sheep) and muscle tissue (sheep cardiac and skeletal) and frozen or PFA fixed muscle tissue (sheep skeletal).

Species Reactivity

All Mammals, Human, Mouse

Applications

ICC, IF

Application Details

Fluorescent imaging of mitochondria. Sample types: live cells, tisue (frozen and 4% paraformaldehyde). Quenching endogenous fluorescence can assist in imaging, refer to the product manual. Not suitable for application on fixed cells. Microscopy: Epifluorescence microscopy: YES; Confocal microscopy: YES; Multiphoton imaging: YES; Infrared spectroscopy: NO; Raman spectroscopy: NO; Visualization: Ex/Em: 405 nm / 600 nm.

Excitation / Emission

405 nm / 600 nm.

Method of Analysis

Fluorescence

Sample Type

Cells, Tissues (live or fixed)

Buffer

DMSO

Physical State

Liquid, ready to dilute

Production Notes

Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of fluorescent isomers. No amount of starting material was detected.

Synonyms

LipoFluor-MR™

Usage Statement

For research use only.

Country of Origin

United States

Expiration

If protected from light, the liquid 10 mM stock solution vials are stable for 12 months from date of receipt, at 2-8°C. A vial should be diluted immediately prior to use and should be used within 24 hours for best results.

Storage

The liquid stock solution vials can be stored up to 12 months from date of receipt at 2-8°C, properly sealed and protected from light. Working solutions should be used within 24 hours and not stored for later use.

Shipping

25°C (ambient)

LipoFluor-MR™ Protocol

Four 0.5 mL microfuge vials containing 5.5 uL/vial of 20 mM stock solution of Lipofluor™ TR-602-Mito in DMSO. 100.8 μg per vial.

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LipoFluor-MR™ Mitochondria Tracing Reagent

SKU: TR-602-Mito

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