LipoFluor-ER2™ Endoplasmic Reticulum Blue Tracing Reagent

Name: LipoFluor-ER2™ Endoplasmic Reticulum Blue Tracing Reagent
Brand: Biosensis
SKU: TR-605-ER2
Price: 297.0 USD
Availability: InStock

Staining in cells with TR-605-ER2 tracing reagent.

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Species Reactivity

All Mammals, Human, Mouse

Applications

ICC, IF

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Data Sheet

SKU: TR-605-ER2

Volume/Size: 4 vials x 5.5 µL per vial

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$297.00

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Product Details

Product Description

LipoFluor-ER2™ is a cell-permeant stain for the endoplasmic reticulum with unique spectral properties, which makes it particularly useful for antibody double- and triple-labelling experiments. LipoFluor-ER2™ is highly compatible with green, red and far-red fluorophore-labelled antibodies and has been successfully used to image the endoplasmic reticulum in human and other mammalian cell lines. It passively diffuses across the plasma membrane into the cell, stains at low concentrations and has minimal cytotoxic effects. This tracing reagent can be used on live and fixed samples, is compatible with other dyes, shows excellent resistance to photobleaching and demonstrates low cytotoxicity.

Target

Mitochondria

Immunogen Species

Human

Specificity

Fluorescent imaging of the endoplasmic reticulum compartment. LipoFluor-ER2™ localizes to the endoplasmic reticulum in a variety of live and fixed eukaryotic cells. It is highly cell permeable and produces fast results with ease and may be used in conjunction with antibody staining. The dye, however, cannot be fixed within the cells with post fixation methods.

Species Reactivity

All Mammals, Human, Mouse

Applications

ICC, IF

Application Details

Fluorescent imaging of the endoplasmic reticulum compartment with emission at a unique blue coloration. Sample types: live cells, fixed cells (4% paraformaldehyde) Microscopy applications: epifluorescence, confocal, multicolour Ex/Em: 405 nm / 500 nm; TR-605-ER2 can be excited by UV (~365 nm) or blue light (405 nm) sources with emissions collected using a wideband pass filter, or narrowband pass filter with an emission range 450-570 nm (Emmax = 500 nm). For confocal microscopy, the dye can be excited by a 400 nm steady state laser, and emission should be collected using a detector suited to blue fluorophores such as DAPI. Alternatively, a spectral detector set for the emission of ER-BTM stain (450-570 nm, Emmax = 500 nm) can be used. Typical working concentration is 50µM (1:1000) dilution in appropriate buffer or cell culture medium. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Excitation / Emission

405 nm / 570 nm

Method of Analysis

Fluorescence

Sample Type

Cells, Tissues (live or fixed)

Buffer

DMSO

Physical State

Liquid, ready to dilute

Production Notes

Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of fluorescent isomers. No amount of starting material was detected.

Synonyms

LipoFluor-ER2™

Usage Statement

For research use only.

Country of Origin

United States

Expiration

If protected from light, the liquid 10 mM stock solution vials are stable for 12 months from date of receipt, at 2-8ºC. A vial should be diluted immediately prior to use and should be used within 24 hours for best results.

Storage

The liquid stock solution vials can be stored up to 12 months from date of receipt at 2-8°C, properly sealed and protected from light. Working solutions should be used within 24 hours and not stored for later use.

Shipping

25°C (ambient)

LipoFluor-ER2™ Protocol

Four 0.5 mL microfuge vials containing 5.5 uL/vial of 50 mM stock solution of Lipofluor™ TR-605-ER2 in DMSO.

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LipoFluor-ER2™ Endoplasmic Reticulum Blue Tracing Reagent

SKU: TR-605-ER2

Product Details

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