LipoFluor-DMN-LD™ is a new cell-permeant stain for the selective staining of lipid droplets (LDs) in various cellular contexts, including live and fixed cells, as well as in three-dimensional speroids, using both single and two-photon excitation.
LipoFluor-DMN-LD™ is a new cell-permeant stain for the selective staining of lipid droplets (LDs) in various cellular contexts, including live and fixed cells, as well as in three-dimensional speroids, using both single and two-photon excitation. The dye's versatility, near-instantaneous cellular uptake, simple compound storage/stability, lower background fluorescence, and high-intensity LD staining make it a valuable tool for rapid resonance scanning and gaining insights into the complex biology of Lds, which has implications for disease states.
Lipid Droplets
Human
LipoFluor-DMN-LD™ is for selective staining of lipid droplets (LDs) in various cellular contexts, including live and fixed cells, as well as in three-dimensional spheroids, using both single and two-photon excitation. The dye's versatility, near-instantaneous cellular uptake, simple compound storage/stability, lower background fluorescence, and high-intensity LD staining make it a valuable tool for rapid resonance scanning and gaining insights into the complex biology of LDs, which has implications for disease states.
All Mammals, Human, Mouse, Rat
ICC, IF
Each vial can be diluted 100X to 2000X depending upon the final working concentration of the dye in the experiment. Typical working concentrations for Lipofluor™ TR-606-LD are DMN-LD at 2μM -10 μM. This concentration was suitable for live cell imaging. It yielded high-quality images of lipid droplets with no evidence of toxicity, demonstrating the efficacy of DMN-LD at this concentration for visualizing lipid droplets in cellular contexts.
403 nm/456 nm
Fluorescence
Live HeLa cells, fixed cells, a three-dimensional spheroid model, and Caco-2 cells cultured a microgravity. Other systems not yet tested
DMSO
Liquid, ready to dilute
Live HeLa cells, fixed cells, a three-dimensional spheroid model, and Caco-2 cells cultured a microgravity. These systems demonstrated the versatility and efficacy of the DMN-LD dye for selective staining of lipid droplets (LDs) in different biological contexts. Additionally, using both single and two-photon excitation further validated the applicability of DMN-LD in diverse imaging settings. The validation of DMN-LD in these test systems emphasizes its potential utility as a valuable tool for studying LD dynamics and cellular processes.
If protected from light, the liquid 10 mM stock solution vials are stable for 12 months from date of receipt, at 2-8°C. A vial should be diluted immediately prior to use and should be used within 24 hours for best results.
The liquid stock solution vials can be stored up to 12 months from date of receipt at 2-8°C, properly sealed and protected from light. Working solutions should be used within 24 hours and not stored for later use.
Four 0.5 mL microfuge vials containing 5.5 uL/vial of 10 mM stock solution of Lipofluor™ TR-606-LD in DMSO.
Bulk Order LipoFluor-DMN-LD™ Lipid Droplets & LAMP1-Positive Vesicle Tracing Reagent
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