Products: Primary Antibodies

Anti-Kv3.2 potassium channel Antibody (N410/17)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-397	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-397	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

Potassium voltage-gated channel subfamily C member 2 or Kv3.2 potassium channel, is a member of the potassium channel, voltage-gated, shaker-related subfamily and the Shaw subfamily (the family includes 4 members Kv3.1-Kv3.4). Kv3.2 is primarily expressed in brain and found in the cortex and hippocampus. It has also been detected in the thalamus and caudate nucleus. Kv3.2 is a membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Diseases associated with KCNC2 include Spinocerebellar Ataxia 13 and Episodic Ataxia, Type 2

Immunogen:

Fusion protein amino acids 474-613 (cytoplasmic C-terminus) of rat Kv3.2a (also known as Potassium voltage-gated channel subfamily C member 2, Kcnc2 and KSHIIIA, accession number P22462-3) Human: 97% identity (137/140 amino acids identical)
Mouse: 86% identity (120/140 amino acids identical)
50% identity with Kv3.1

Protein Name(s):

Potassium voltage-gated channel subfamily C member 2 (Potassium channel voltage-gated Shaw-related subfamily C member 2) (Shaw-like potassium channel) (Voltage-gated potassium channel subunit Kv3.2)

Gene Name(s):

Kcnc2

Antibody Registry ID:

Purified: AB_2491084
TC Supernatant: AB_2341104

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

80-100 kDa (varies due to post-translational modifications)

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.