ICC IHC WB
Fusion protein amino acids 717-907 (cytoplasmic C-terminus) of rat Kv2.2 long isoform (also known as Potassium voltage-gated channel subfamily B member 2, Kcnb2 and CDRK, accession number NP_446452.2)
Mouse: 94% identity (180/191 amino acids identical)
Human: 84% identity (161/191 amino acids identical)
50% identity with Kv2.1 and other Kv2 channels
100% identity with Kv2.2 short isoform for first 47 amino acids (ENRGSAPQTPPSTARPLPVTTADFPLTTPQHMSTILLEEALPQGQRP)
Does not cross-react with Kv2.2 short isoform Does not cross-react with Kv2.1
Potassium voltage-gated channel subfamily B member 2 (CDRK) (Voltage-gated potassium channel subunit Kv2.2)
TC Supernatant: AB_2315867
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
Citations and References
- Bishop HI1, Cobb MM1, Kirmiz M1, Parajuli LK2,3, Mandikian D1, Philp AM1, Melnik M1, Kuja-Panula J4, Rauvala H4, Shigemoto R3, Murray KD1,2, Trimmer JS1,5. (2018), 'Kv2 Ion Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons..' Front Mol Neurosci. 10.3389/fnmol.2018.00001.
- Bishop HI1, Guan D2, Bocksteins E3, Parajuli LK4, Murray KD4, Cobb MM1, Misonou H5, Zito K6, Foehring RC2, Trimmer JS7.. (2015), 'Distinct Cell- and Layer-Specific Expression Patterns and Independent Regulation of Kv2 Channel Subtypes in Cortical Pyramidal Neurons..' J Neurosci. 10.1523/JNEUROSCI.1897-15.2015.